Source:http://linkedlifedata.com/resource/pubmed/id/10233761
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1999-6-18
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| pubmed:abstractText |
Anti-histone antibodies have been detected in the sera of patients with various autoimmune diseases. The existence of anti-histone antibodies in patients with polymyositis/dermatomyositis, however, has not been reported. We found anti-histone antibodies in eight (17%) of 46 sera from patients with polymyositis/dermatomyositis by an enzyme-linked immunosorbent assay. One serum was positive for both IgG anti-histone antibodies and IgM anti-histone antibodies. Six sera were positive only for IgG anti-histone antibodies. One serum was positive only for IgM anti-histone antibodies. An indirect immunofluorescence analysis using HEp-2 cells as the substrate showed that all sera positive for anti-histone antibodies produced homogeneous nuclear fluorescence. This immunofluorescence pattern disappeared after absorption of anti-histone activity with total histones. An immunoblotting analysis demonstrated that the anti-histone antibodies were predominantly directed against histone H1 in all seven sera with IgG anti-histone antibodies. Weak reactivity with H2B and H4 were also found in three sera from the patients with polymyositis/dermatomyositis. Sera from two patients with polymyositis/dermatomyositis displayed anti-H2A and H3 activity. One of the two sera showed IgM anti-histone antibodies in the enzyme-linked immunosorbent assay reacted with H1, H2A, H2B, H3, and H4, whereas the other serum reacted with no fractions of total histones. The activity of anti-histone antibodies disappeared in immunoblotting after absorption with total histones. All of the patients with anti-histone antibodies were free from lung fibrosis or internal malignancies. Thus, our data indicate that the presence of anti-histone antibodies is classified as one of the serologic abnormalities observed in polymyositis/dermatomyositis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-202X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
112
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
711-5
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10233761-Adolescent,
pubmed-meshheading:10233761-Adult,
pubmed-meshheading:10233761-Aged,
pubmed-meshheading:10233761-Antibody Specificity,
pubmed-meshheading:10233761-Autoantibodies,
pubmed-meshheading:10233761-Cell Line,
pubmed-meshheading:10233761-Dermatomyositis,
pubmed-meshheading:10233761-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10233761-Female,
pubmed-meshheading:10233761-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:10233761-Histones,
pubmed-meshheading:10233761-Humans,
pubmed-meshheading:10233761-Immunoblotting,
pubmed-meshheading:10233761-Immunoglobulin G,
pubmed-meshheading:10233761-Immunoglobulin M,
pubmed-meshheading:10233761-Male,
pubmed-meshheading:10233761-Middle Aged,
pubmed-meshheading:10233761-Polymyositis
|
| pubmed:year |
1999
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| pubmed:articleTitle |
Prevalence and antigen specificity of anti-histone antibodies in patients with polymyositis/dermatomyositis.
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| pubmed:affiliation |
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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