Source:http://linkedlifedata.com/resource/pubmed/id/10228021
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
1999-5-20
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| pubmed:abstractText |
MHC class Ia-deficient mice (H2 Kb-/- Db-/-) inoculated with the intracellular pathogen Listeria monocytogenes (LM) displayed a three- to fourfold expansion of splenic CD8+ T cells 6 days following infection. Culture of these spleen cells in vitro gave rise to CTL that recognized LM-infected target cells and were restricted by the class Ib molecules, Qa1b and M3. Exposure of target cells to heat-killed LM (HKLM) rather than live bacteria did not result in CTL-mediated lysis. Target cells pulsed with three LM peptides known to bind M3, f-MIGWII, f-MIVTLF, and f-MIVIL, were recognized by effector cells from both B6 and Kb-/- Db-/- animals. In vivo analysis showed that B6 and Kb-/- Db-/- mice clear LM from the spleen and liver rapidly with similar kinetics, whereas TAP.1-/- mice, which are deficient in class Ia and Ib molecules, clear LM slowly upon infection. To establish the in vivo role of CD8+ T cells in Kb-/- Db-/- animals, we showed that depletion of such cells from the spleens of immune mice prevented the adoptive transfer of protective immunity to syngeneic recipients. Spleen cells from Kb-/- Db-/- mice were also capable of generating responses directed against syngeneic as well as allogeneic class Ia molecules in vitro. Thus, class Ia-deficient animals have a CD8+ T cell repertoire capable of recognizing both class Ia and class Ib molecules and can generate protective immunity to LM.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/H-2K(K) antigen,
http://linkedlifedata.com/resource/pubmed/chemical/H-2Kb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Q surface antigens
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
|
| pubmed:volume |
162
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5429-36
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:10228021-Animals,
pubmed-meshheading:10228021-Antigen Presentation,
pubmed-meshheading:10228021-Antigens, Bacterial,
pubmed-meshheading:10228021-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10228021-Epitopes, T-Lymphocyte,
pubmed-meshheading:10228021-H-2 Antigens,
pubmed-meshheading:10228021-Histocompatibility Antigens Class I,
pubmed-meshheading:10228021-Listeria monocytogenes,
pubmed-meshheading:10228021-Listeriosis,
pubmed-meshheading:10228021-Liver,
pubmed-meshheading:10228021-Lymphocyte Activation,
pubmed-meshheading:10228021-Lymphocyte Count,
pubmed-meshheading:10228021-Mice,
pubmed-meshheading:10228021-Mice, Inbred BALB C,
pubmed-meshheading:10228021-Mice, Inbred C57BL,
pubmed-meshheading:10228021-Mice, Knockout,
pubmed-meshheading:10228021-Spleen,
pubmed-meshheading:10228021-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10228021-Virulence
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| pubmed:year |
1999
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| pubmed:articleTitle |
Response to Listeria monocytogenes in mice lacking MHC class Ia molecules.
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| pubmed:affiliation |
Immunology Graduate Program, Center for Immunology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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