Linked Life Data

10227591

Source:http://linkedlifedata.com/resource/pubmed/id/10227591

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1999-5-18
pubmed:abstractText
Recent data have demonstrated that caveolin, a major structural protein of caveolae, inhibits the function of molecules involved in cAMP signaling such as adenylyl cyclase. We examined the effect of cAMP signal on the expressions of caveolin subtypes using rat cardiac myoblasts (H9C2 cells) and smooth muscle cells (RASMC), which express caveolin subtypes. Treatment of RASMC and H9C2 cells with forskolin, an adenylyl cyclase stimulator, decreased caveolin-1 mRNA levels in a dose-dependent manner. Time course studies showed a time-dependent decrease of caveolin-1 mRNA levels in H9C2 cells (after 6 hours) while caveolin-1 mRNA levels in RASMC showed a biphasic response, i.e., an initial increase (within 3 hours) and a later decrease (after 3 hours). Similar biphasic changes were observed when RASMC was treated with IBMX, a phosphodiesterase inhibitor. The levels of caveolin-1 and -3 proteins were also decreased by forskolin treatment, but only after 60-72 hours in RASMC and 24-36 hours in H9C2 cells. In contrast, the expression of caveolin-2 remained similar in both cells and decreased to a small degree after prolonged treatment. Therefore, the expression of caveolin is downregulated by cAMP signal in a caveolin subtype-dependent manner.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1349-57
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Downregulation of caveolin expression by cAMP signal.
pubmed:affiliation
Cardiovascular & Pulmonary Research Institute, Allegheny University of the Health Sciences, Pittsburgh, PA 15212, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't