Linked Life Data

10223205

Source:http://linkedlifedata.com/resource/pubmed/id/10223205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-5-20
pubmed:abstractText
To investigate how various levels of exposure affect the metabolic activation pathways of benzene in humans and to examine the relationship between urinary metabolites and other biological markers, we have developed a sensitive and specific liquid chromatographic-tandem mass spectrometric assay for simultaneous quantitation of urinary S-phenylmercapturic acid (S-PMA) and trans,trans-muconic acid (t,t-MA). The assay involves spiking urine samples with [13C6]S-PMA and [13C6]t,t-MA as internal standards and clean up of samples by solid-phase extraction with subsequent analysis by liquid chromatography coupled with electrospray-tandem mass spectrometry-selected reaction monitoring (LC-ES-MS/MS-SRM) in the negative ionization mode. The efficacy of this assay was evaluated in human urine specimens from smokers and non-smokers as the benzene-exposed and non-exposed groups. The coefficient of variation of runs on different days (n = 8) for S-PMA was 7% for the sample containing 9.4 microg S-PMA/l urine, that for t,t-MA was 10% for samples containing 0.07 mg t,t-MA/l urine. The mean levels of urinary S-PMA and t,t-MA in smokers were 1.9-fold (P = 0.02) and 2.1-fold (P = 0.03) higher than those in non-smokers. The mean urinary concentration (+/-SE) was 9.1 +/- 1.7 microg S-PMA/g creatinine [median 5.8 microg/g, ranging from not detectable (1 out of 28) to 33.4 microg/g] among smokers. In non-smokers' urine the mean concentration was 4.8 +/- 1.1 microg S-PMA/g creatinine (median 3.6 microg/g, ranging from 1.0 to 19.6 microg/g). For t,t-MA in smokers' urine the mean (+/-SE) was 0.15 +/- 0.03 mg/g creatinine (median 0.11 mg/ g, ranging from 0.005 to 0.34 mg/g); the corresponding mean value for t,t-MA concentration in non-smokers' urine was 0.07 +/- 0.02 mg/g creatinine [median 0.03 mg/g, ranging from undetectable (1 out of 18) to 0.48 mg/g]. There was a correlation between S-PMA and t,t-MA after logarithmic transformation (r = 0.41, P = 0.005, n = 46).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
719-26
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10223205-Acetylcysteine, pubmed-meshheading:10223205-Animals, pubmed-meshheading:10223205-Benzene, pubmed-meshheading:10223205-Biological Markers, pubmed-meshheading:10223205-Biotransformation, pubmed-meshheading:10223205-Calibration, pubmed-meshheading:10223205-Chromatography, High Pressure Liquid, pubmed-meshheading:10223205-Environmental Exposure, pubmed-meshheading:10223205-Evaluation Studies as Topic, pubmed-meshheading:10223205-Humans, pubmed-meshheading:10223205-Mass Spectrometry, pubmed-meshheading:10223205-Rats, pubmed-meshheading:10223205-Rats, Inbred F344, pubmed-meshheading:10223205-Reference Standards, pubmed-meshheading:10223205-Reproducibility of Results, pubmed-meshheading:10223205-Sensitivity and Specificity, pubmed-meshheading:10223205-Smoking, pubmed-meshheading:10223205-Sorbic Acid, pubmed-meshheading:10223205-Urinalysis
pubmed:year
1999
pubmed:articleTitle
Determination of the urinary benzene metabolites S-phenylmercapturic acid and trans,trans-muconic acid by liquid chromatography-tandem mass spectrometry.
pubmed:affiliation
Naylor Dana Institute, American Health Foundation, Valhalla, NY 10595, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't