Linked Life Data

10219298

Source:http://linkedlifedata.com/resource/pubmed/id/10219298

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-5-20
pubmed:abstractText
Differential mRNA display was used to detect differences in gene expression between mock-infected and Cryptosporidium parvum-infected human adenocarcinoma cells. A reproducible band present only in C. parvum-infected cells, ddHC-10 was isolated and cloned. Northern blot analysis was used to confirm the differential expression of the HC-10 mRNA. As differential mRNA display does not differentiate between parasite and host mRNAs, Southern blot analysis was used to demonstrate that ddHC-10 represented a C. parvum gene. Northern blot analysis demonstrated that HC-10 mRNA is expressed by sporozoites prior to invasion of host cells. Screening of a C. parvum genomic library identified 2 different genomic clones, HC-10-13C and HC-10-6C. The combined genomic sequence contained a predicted open reading frame of 2,952 base pairs (bp), coding for a protein of 984 amino acids with a predicted molecular weight of approximately 106 kDa. Reverse transcription polymerase chain reaction mapping of the HC-10 transcript demonstrated that the HC-10 gene lacks introns, and the approximately 4,789-bp mRNA contains relatively large 5' (approximately 1,390-bp) and 3' (approximately 440-bp) untranslated regions. The predicted polypeptide contained a high proportion of polar amino acids, with the most abundant amino acids being serine (10.5%), threonine (9.8%), and cysteine (7.6%). The C-terminal region of the predicted polypeptide is characterized by a threonine-rich region containing multiple repeats of the sequence TTTTRP. This repeat motif is similar to that found in the mucin-like genes of vertebrates and lower eukaryotes that have been shown to play important roles in cell-cell interactions in multicellular organisms and invasion of host cells by unicellular parasites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3395
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-20
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10219298-Amino Acid Sequence, pubmed-meshheading:10219298-Animals, pubmed-meshheading:10219298-Blotting, Northern, pubmed-meshheading:10219298-Blotting, Southern, pubmed-meshheading:10219298-Cattle, pubmed-meshheading:10219298-Cryptosporidium parvum, pubmed-meshheading:10219298-DNA, Complementary, pubmed-meshheading:10219298-Gene Expression Regulation, Developmental, pubmed-meshheading:10219298-Genes, Protozoan, pubmed-meshheading:10219298-Glycoproteins, pubmed-meshheading:10219298-Host-Parasite Interactions, pubmed-meshheading:10219298-Humans, pubmed-meshheading:10219298-Molecular Sequence Data, pubmed-meshheading:10219298-Phosphoproteins, pubmed-meshheading:10219298-Protozoan Proteins, pubmed-meshheading:10219298-RNA, Messenger, pubmed-meshheading:10219298-RNA, Protozoan, pubmed-meshheading:10219298-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10219298-Tumor Cells, Cultured
pubmed:year
1999
pubmed:articleTitle
Differential mRNA display cloning and characterization of a Cryptosporidium parvum gene expressed during intracellular development.
pubmed:affiliation
University of Minnesota, St. Paul 55108, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't