Source:http://linkedlifedata.com/resource/pubmed/id/10216082
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
1999-5-18
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pubmed:abstractText |
Better understanding of hemostasis will be possible by the identification of new lineage-specific stimuli that regulate platelet formation. We describe a novel functional megakaryocyte receptor that belongs to a family of ionotropic glutamate receptors of the N-methyl-D-aspartate (NMDA) subtype responsible for synaptic neurotransmission in the central nervous system (CNS). Northern blotting and reverse-transcriptase polymerase chain reaction (RT-PCR) studies identified expression of NMDAR1 and NMDAR2D type subunit mRNA in rat marrow, human megakaryocytes, and MEG-01 clonal megakaryoblastic cells. Immunohistochemistry and in vivo autoradiographic binding of the NMDA receptor-specific antagonist MK-801 confirmed that megakaryocytes expressed open channel-forming NMDA receptors in vivo. Western blots indicated that megakaryocyte NMDAR1 was either unglycosylated or only glycosylated to low levels, and of identical size to CNS-type NMDAR1 after deglycosylation with endoglycosidase F/peptide-N-glycosidase F. In functional studies, we demonstrated that NMDA receptor activity was necessary for phorbol myristate acetate (PMA)-induced differentiation of megakaryoblastic cells; NMDA receptor blockade by specific antagonists significantly inhibited PMA-mediated increases in cell size, CD41 expression, and adhesion of MEG-01 cells. These results provide evidence for a novel pathway by which megakaryocytopoiesis and platelet production may be regulated.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/NMDA receptor A1,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Glycoprotein GPIIb-IIIa...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2876-83
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:10216082-Animals,
pubmed-meshheading:10216082-Bone Marrow Cells,
pubmed-meshheading:10216082-Cells, Cultured,
pubmed-meshheading:10216082-Clone Cells,
pubmed-meshheading:10216082-Fetal Blood,
pubmed-meshheading:10216082-Flow Cytometry,
pubmed-meshheading:10216082-Humans,
pubmed-meshheading:10216082-Immunohistochemistry,
pubmed-meshheading:10216082-Infant, Newborn,
pubmed-meshheading:10216082-Megakaryocytes,
pubmed-meshheading:10216082-Platelet Glycoprotein GPIIb-IIIa Complex,
pubmed-meshheading:10216082-Rats,
pubmed-meshheading:10216082-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:10216082-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10216082-Tetradecanoylphorbol Acetate
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pubmed:year |
1999
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pubmed:articleTitle |
Expression of a functional N-methyl-D-aspartate-type glutamate receptor by bone marrow megakaryocytes.
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pubmed:affiliation |
Department of Biology, University of York, York, United Kingdom. pg5@york.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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