Source:http://linkedlifedata.com/resource/pubmed/id/10215024
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1999-9-2
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| pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF065168,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF065600,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF065601,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U89152,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U89153
|
| pubmed:abstractText |
A new variant-specific surface protein gene locus (vsp417-4) of Giardia intestinalis is described. Vsp417-4 represents the fourth member of a gene subfamily that is based on a previously described gene, tsa417 ( =vsp417-1). The new locus was detected by characterising DNA amplified in polymerase chain reactions from the 3' ends of divergent homologues (vsp417-4(A-I), vsp417-4(A-II)) found respectively in isolates belonging to the genetic Assemblage A/Group I ('A-I') and Assemblage A/Group II ('A-II') subtypes of G. intestinalis. The complete vsp417-4(A-I) gene was isolated on a 6.2-kb HindIII fragment by screening a genomic DNA library prepared from a type A-I isolate, Ad-1/C7. The deduced polypeptide (VSP417-4(A-I); 709 amino acids, Mr 72662) has properties characterising it as a Giardia variant-specific surface protein, namely a high cysteine content (11.85 mol%), 29 copies of the four amino-acid 'CXXC' motif, and conserved N-terminal signal peptide and C-terminal hydrophobic (membrane-spanning) segments--the latter terminating with the invariant, hydrophilic motif '-CRGKA'. An extended polyadenylation signal sequence (CTTAGRTAGTAAAY), which appears to be a characteristic feature of VSP genes in Giardia, is situated immediately beyond the stop codon. VSP417-4(A-I) shares 87% sequence identity with VSP417-4(A-II) over its C-terminal 235 amino acids, but only 57-58% identity with VSP417-1, VSP417-2 and VSP417-3 which are encoded by other vsp417 family genes identified in these genotypes. Southern hybridisations, using probes derived from the 5' segment of vsp417-4(A-I), indicated the presence of at least five to six closely related loci in both type A-I and type A-II isolates.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0166-6851
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
99
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
55-68
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10215024-Alleles,
pubmed-meshheading:10215024-Amino Acid Sequence,
pubmed-meshheading:10215024-Animals,
pubmed-meshheading:10215024-Antigens, Protozoan,
pubmed-meshheading:10215024-Antigens, Surface,
pubmed-meshheading:10215024-Blotting, Southern,
pubmed-meshheading:10215024-Evolution, Molecular,
pubmed-meshheading:10215024-Genes, Protozoan,
pubmed-meshheading:10215024-Giardia lamblia,
pubmed-meshheading:10215024-Humans,
pubmed-meshheading:10215024-Molecular Sequence Data,
pubmed-meshheading:10215024-Multigene Family,
pubmed-meshheading:10215024-Phylogeny,
pubmed-meshheading:10215024-Polymerase Chain Reaction,
pubmed-meshheading:10215024-Protozoan Proteins,
pubmed-meshheading:10215024-Restriction Mapping,
pubmed-meshheading:10215024-Sequence Alignment,
pubmed-meshheading:10215024-Sequence Analysis, DNA
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| pubmed:year |
1999
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| pubmed:articleTitle |
A new locus (vsp417-4) belonging to the tsa417-like subfamily of variant-specific surface protein genes in Giardia intestinalis.
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| pubmed:affiliation |
Department of Microbiology and Immunology, The University of Adelaide, SA, Australia. pey@microb.adelaide.edu.au
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|