| pubmed-article:10210284 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C0814999 | lld:lifeskim |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C1326205 | lld:lifeskim |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:10210284 | lifeskim:mentions | umls-concept:C0171608 | lld:lifeskim |
| pubmed-article:10210284 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:10210284 | pubmed:dateCreated | 1999-4-29 | lld:pubmed |
| pubmed-article:10210284 | pubmed:abstractText | Microcolin A (Mic-1), a marine-derived compound, has been shown to be a novel antiproliferative and immunosuppressive agent. We investigated the ability of Mic-1 and its chemosynthetic analog, microcolin A3 (Mic-3), to induce apoptosis in murine thymocytes. Following incubation of the cells with Mic-1 (10-100 nM) or Mic-3 (10-100 nM), internucleosomal DNA fragmentation in apoptotic cells was detected by agarose gel electrophoresis and the diphenylamine (DPA) assay; the presence of hypodiploid nuclei assessed by propidium iodide (PI) staining; and the percentages of apoptotic and necrotic cells quantified by morphological observation and fluorescein labeled annexin-V binding. Our results show that both Mic-1 and Mic-3 are potent inducers of apoptosis in thymocytes depending on drug concentration and time of exposure, with Mic-3 being more potent than Mic-1 in the induction of apoptosis. Furthermore, flow cytometric analysis using monoclonal antibodies specific to thymocyte subpopulations showed that the proportion of the early immature CD4+ CD8+ T-cell subpopulation in thymocytes was selectively decreased by both agents with a corresponding increase of other subpopulations, indicating that CD4+ CD8+ T cells are the most likely targets of Mic-1 and Mic-3. These in vitro results suggest that the antiproliferative and immunosuppressive properties of both compounds are possibly associated with apoptosis-inducing events and imply that they may have additional potential value as antineoplastic agents. | lld:pubmed |
| pubmed-article:10210284 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10210284 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10210284 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10210284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10210284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10210284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10210284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10210284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10210284 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10210284 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:10210284 | pubmed:author | pubmed-author:LongleyR ERE | lld:pubmed |
| pubmed-article:10210284 | pubmed:author | pubmed-author:ZhangL HLH | lld:pubmed |
| pubmed-article:10210284 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10210284 | pubmed:volume | 64 | lld:pubmed |
| pubmed-article:10210284 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10210284 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10210284 | pubmed:pagination | 1013-28 | lld:pubmed |
| pubmed-article:10210284 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
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| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:meshHeading | pubmed-meshheading:10210284... | lld:pubmed |
| pubmed-article:10210284 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10210284 | pubmed:articleTitle | Induction of apoptosis in mouse thymocytes by microcolin A and its synthetic analog. | lld:pubmed |
| pubmed-article:10210284 | pubmed:affiliation | Division of Biomedical Marine Research, Harbor Branch Oceanographic Institution, Inc., Fort Pierce, FL 34946, USA. | lld:pubmed |
| pubmed-article:10210284 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10210284 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10210284 | lld:pubmed |
