Source:http://linkedlifedata.com/resource/pubmed/id/10210284
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1999-4-29
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pubmed:abstractText |
Microcolin A (Mic-1), a marine-derived compound, has been shown to be a novel antiproliferative and immunosuppressive agent. We investigated the ability of Mic-1 and its chemosynthetic analog, microcolin A3 (Mic-3), to induce apoptosis in murine thymocytes. Following incubation of the cells with Mic-1 (10-100 nM) or Mic-3 (10-100 nM), internucleosomal DNA fragmentation in apoptotic cells was detected by agarose gel electrophoresis and the diphenylamine (DPA) assay; the presence of hypodiploid nuclei assessed by propidium iodide (PI) staining; and the percentages of apoptotic and necrotic cells quantified by morphological observation and fluorescein labeled annexin-V binding. Our results show that both Mic-1 and Mic-3 are potent inducers of apoptosis in thymocytes depending on drug concentration and time of exposure, with Mic-3 being more potent than Mic-1 in the induction of apoptosis. Furthermore, flow cytometric analysis using monoclonal antibodies specific to thymocyte subpopulations showed that the proportion of the early immature CD4+ CD8+ T-cell subpopulation in thymocytes was selectively decreased by both agents with a corresponding increase of other subpopulations, indicating that CD4+ CD8+ T cells are the most likely targets of Mic-1 and Mic-3. These in vitro results suggest that the antiproliferative and immunosuppressive properties of both compounds are possibly associated with apoptosis-inducing events and imply that they may have additional potential value as antineoplastic agents.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/microcolin A
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pubmed:status |
MEDLINE
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pubmed:issn |
0024-3205
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1013-28
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10210284-Animals,
pubmed-meshheading:10210284-Antineoplastic Agents,
pubmed-meshheading:10210284-Apoptosis,
pubmed-meshheading:10210284-DNA Fragmentation,
pubmed-meshheading:10210284-Immunosuppressive Agents,
pubmed-meshheading:10210284-Male,
pubmed-meshheading:10210284-Mice,
pubmed-meshheading:10210284-Mice, Inbred C57BL,
pubmed-meshheading:10210284-Necrosis,
pubmed-meshheading:10210284-Oligopeptides,
pubmed-meshheading:10210284-Pyrrolidines,
pubmed-meshheading:10210284-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
Induction of apoptosis in mouse thymocytes by microcolin A and its synthetic analog.
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pubmed:affiliation |
Division of Biomedical Marine Research, Harbor Branch Oceanographic Institution, Inc., Fort Pierce, FL 34946, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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