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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017968,
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0205148,
umls-concept:C0332307,
umls-concept:C0348055,
umls-concept:C1171362,
umls-concept:C1514562,
umls-concept:C1515670,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
5
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pubmed:dateCreated |
1999-7-16
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pubmed:databankReference |
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pubmed:abstractText |
Five different V3 domains of HIV-1 gp120 were expressed on the surface of the gram-positive bacterium Streptococcus gordonii, a model live vector for vaccine delivery. Sera of HIV-1-infected individuals and human monoclonal antibodies specifically recognized the gp120 sequences on the bacterial surface. Recombinant V3 from the reference HIV-1 strain MN was also shown to retain a conformation that allowed reaction with a conformation-specific monoclonal antibody. A V3-specific serum antibody response was detected in mice immunized both by subcutaneous injection and by vaginal colonization. V3-specific IgG2a antibodies, suggestive of a Th1 response, were found in the sera of mice colonized by recombinant bacteria.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HIV envelope protein gp120 (305-321),
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/streptococcal M protein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0889-2229
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pubmed:author |
pubmed-author:BraccoJJ,
pubmed-author:LozziLL,
pubmed-author:MaggiTT,
pubmed-author:MancaFF,
pubmed-author:MedagliniDD,
pubmed-author:OggioniM RMR,
pubmed-author:PeruzziFF,
pubmed-author:PozziGG,
pubmed-author:RomaniRR,
pubmed-author:ValensinP EPE,
pubmed-author:ZazziMM
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10195755-Amino Acid Sequence,
pubmed-meshheading:10195755-Animals,
pubmed-meshheading:10195755-Antigens, Bacterial,
pubmed-meshheading:10195755-Bacterial Outer Membrane Proteins,
pubmed-meshheading:10195755-Bacterial Proteins,
pubmed-meshheading:10195755-Blotting, Western,
pubmed-meshheading:10195755-Carrier Proteins,
pubmed-meshheading:10195755-DNA, Viral,
pubmed-meshheading:10195755-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10195755-Female,
pubmed-meshheading:10195755-Fluorescent Antibody Technique,
pubmed-meshheading:10195755-Genetic Vectors,
pubmed-meshheading:10195755-HIV Antibodies,
pubmed-meshheading:10195755-HIV Antigens,
pubmed-meshheading:10195755-HIV Envelope Protein gp120,
pubmed-meshheading:10195755-HIV Infections,
pubmed-meshheading:10195755-HIV-1,
pubmed-meshheading:10195755-Humans,
pubmed-meshheading:10195755-Immunization,
pubmed-meshheading:10195755-Immunoglobulin G,
pubmed-meshheading:10195755-Mice,
pubmed-meshheading:10195755-Mice, Inbred BALB C,
pubmed-meshheading:10195755-Molecular Sequence Data,
pubmed-meshheading:10195755-Peptide Fragments,
pubmed-meshheading:10195755-Recombinant Fusion Proteins,
pubmed-meshheading:10195755-Recombinant Proteins,
pubmed-meshheading:10195755-Streptococcus
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pubmed:year |
1999
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pubmed:articleTitle |
Antigenicity and immunogenicity of the V3 domain of HIV type 1 glycoprotein 120 expressed on the surface of Streptococcus gordonii.
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pubmed:affiliation |
Dipartimento di Biologia Molecolare, Università di Siena, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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