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rdf:type |
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lifeskim:mentions |
umls-concept:C0020971,
umls-concept:C0025663,
umls-concept:C0039195,
umls-concept:C0205263,
umls-concept:C0205314,
umls-concept:C0206558,
umls-concept:C0679622,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1824884,
umls-concept:C2608085,
umls-concept:C2911692
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pubmed:issue |
9-10
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pubmed:dateCreated |
1999-6-2
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pubmed:abstractText |
DNA molecules complexed with an asialoglycoprotein-polycation conjugate, consisting of asialoorosomucoid (ASOR) coupled to poly-L-lysine, can enter hepatocytes which bear receptors for ASOR. We used this receptor-mediated DNA delivery system to deliver plasmid DNA encoding glycoprotein D (gD) of herpes simplex virus type 1 to ASOR-positive cells. Maximum expression of gD protein was seen at 3 days after injection of this preparation in approximately 13% of cells from BALB/c mice [hepatocytes from mice injected intravenously (i.v.) or peritoneal exudate cells from mice injected intraperitoneally (i.p.)]. In comparison with mice injected with either the plasmid vector alone or the gD-containing plasmid uncomplexed to ASOR, mice immunized with gD-containing plasmid complexed with ASOR-poly-L-lysine induced marked antigen-specific CTL responses. BALB/c mice immunized with gD-DNA developed a T-cell-mediated CTL response against target cells expressing gD and MHC class II glycoproteins, but not against cells expressing only gD and MHC class I molecules. In C3H mice, gD-DNA induced a T-cell-mediated CTL response against target cells expressing gD and class I MHC molecules. Serum anti-gD antibody in low titers were produced in both strains of mice. DNA complexed with ASOR-poly-L-lysine induced CTL responses in mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Asialoglycoprotein Receptor,
http://linkedlifedata.com/resource/pubmed/chemical/Asialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Orosomucoid,
http://linkedlifedata.com/resource/pubmed/chemical/Polylysine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/asialoorosomucoid,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein D, Human herpesvirus 1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1091-9
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:10195619-Animals,
pubmed-meshheading:10195619-Antibodies, Viral,
pubmed-meshheading:10195619-Antigens, CD4,
pubmed-meshheading:10195619-Asialoglycoprotein Receptor,
pubmed-meshheading:10195619-Asialoglycoproteins,
pubmed-meshheading:10195619-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10195619-Female,
pubmed-meshheading:10195619-Hemagglutinins, Viral,
pubmed-meshheading:10195619-Liver,
pubmed-meshheading:10195619-Mice,
pubmed-meshheading:10195619-Mice, Inbred BALB C,
pubmed-meshheading:10195619-Mice, Inbred C3H,
pubmed-meshheading:10195619-Orosomucoid,
pubmed-meshheading:10195619-Plasmids,
pubmed-meshheading:10195619-Polylysine,
pubmed-meshheading:10195619-Receptors, Cell Surface,
pubmed-meshheading:10195619-Simplexvirus,
pubmed-meshheading:10195619-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10195619-Transfection,
pubmed-meshheading:10195619-Vaccination,
pubmed-meshheading:10195619-Viral Envelope Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
A novel immunization method to induce cytotoxic T-lymphocyte responses (CTL) against plasmid-encoded herpes simplex virus type-1 glycoprotein D.
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pubmed:affiliation |
Department of Microbiology, Wright State University, Dayton, OH 45435, USA.
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pubmed:publicationType |
Journal Article
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