Linked Life Data

10190314

Source:http://linkedlifedata.com/resource/pubmed/id/10190314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-4-15
pubmed:abstractText
Human hepatocellular carcinomas (HCC) are known to frequently exhibit clear-cell or fatty change. The expression of three enzymes related to fatty acid metabolism, the peroxisomal bifunctional enzyme (enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase, BE), cytosolic carbonyl reductase (CR) and the alpha-class glutathione S-transferase (GST) was investigated immunohistochemically in 45 HCC samples, to examine their relevance to this phenomenon and to antioxidant cellular defence. The tumour sizes ranged from 3 mm to 37 mm in diameter (mean 19 mm). Of 8 highly differentiated carcinomas (Edmondson's grade 1), 5 and 6 showed positive staining for BE and CR respectively, like the surrounding non-hepatoma tissues. Of 37 Edmondson's grade II-IV lesions, 31 exhibited negative or only weakly positive staining for both enzymes, as compared with the surrounding tissues. The combined rates for weakly positive and negative staining for BE or CR were proportional to the degree of dedifferentiation. However, 3 of 26 grade III tumours showed enhanced staining. Intensities of staining for CR were in accordance with those for BE in 40 of the total of 45 HCC. Immunoblot analysis also demonstrated concerted alteration of the two enzymes in carcinoma tissues. The staining of the alpha-class GST was hardly changed in Edmondson's grade I and II cases but was decreased in 24 of 31 grade III and IV lesions. The great majority of the BE-negative carcinomas did not demonstrate fatty or clear-cell change. These results suggested that BE and CR might be possible markers for the analysis of multistage hepatocarcinogenesis but that decrease or loss was not reflected in increased fat storage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0171-5216
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10190314-3-Hydroxyacyl CoA Dehydrogenases, pubmed-meshheading:10190314-Adult, pubmed-meshheading:10190314-Aged, pubmed-meshheading:10190314-Alcohol Oxidoreductases, pubmed-meshheading:10190314-Carcinoma, Hepatocellular, pubmed-meshheading:10190314-Cytosol, pubmed-meshheading:10190314-Enoyl-CoA Hydratase, pubmed-meshheading:10190314-Female, pubmed-meshheading:10190314-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10190314-Glutathione Transferase, pubmed-meshheading:10190314-Humans, pubmed-meshheading:10190314-Immunoblotting, pubmed-meshheading:10190314-Immunohistochemistry, pubmed-meshheading:10190314-Isomerases, pubmed-meshheading:10190314-Liver Neoplasms, pubmed-meshheading:10190314-Male, pubmed-meshheading:10190314-Middle Aged, pubmed-meshheading:10190314-Multienzyme Complexes, pubmed-meshheading:10190314-Oxidative Stress
pubmed:year
1999
pubmed:articleTitle
Decreased expression of the peroxisomal bifunctional enzyme and carbonyl reductase in human hepatocellular carcinomas.
pubmed:affiliation
Second Department of Pathology, Hirosaki University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't