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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1977-3-31
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pubmed:abstractText |
The poly(A)-specific endoribonuclease IV produces oligo(A) fragments of a chain length of 10 AMP nucleotides. One enzyme molecule performs 1700 cleavages per min; the cleavages occur randomly. The endoribonuclease IV is a nuclear enzyme which is present in the oviduct of quails in a concentration of 40 000 enzyme molecules per cell. Poly(A) segments on mRNA are selectively hydrolyzed by endoribonuclease IV; the poly(A)-free part of the RNA is not affected. After incubation with the enzyme, a residual oligo(A) stretch of 5 AMP nucleotides remains on poly(A)-rich RNA. The endoribonuclease IV does not disintegrate the polyribosomal complex after incubation in vitro and the enzyme has also no influence on the translational capacity of a cell-free protein-synthesizing system.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
249-58
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:1009929-Animals,
pubmed-meshheading:1009929-Cell Nucleus,
pubmed-meshheading:1009929-Endonucleases,
pubmed-meshheading:1009929-Female,
pubmed-meshheading:1009929-Kinetics,
pubmed-meshheading:1009929-Molecular Weight,
pubmed-meshheading:1009929-Oligoribonucleotides,
pubmed-meshheading:1009929-Oviducts,
pubmed-meshheading:1009929-Plants,
pubmed-meshheading:1009929-Poly A,
pubmed-meshheading:1009929-Polyribosomes,
pubmed-meshheading:1009929-Quail,
pubmed-meshheading:1009929-Ribonucleases,
pubmed-meshheading:1009929-Subcellular Fractions
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pubmed:year |
1976
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pubmed:articleTitle |
Endoribonuclease IV. 2. Further investigation on the specificity.
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pubmed:publicationType |
Journal Article
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