Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-5-20
pubmed:abstractText
Insulin-like growth factor 1 (IGF1) has been proposed as a "G1-progression factor" and as a mediator of estradiol's (E2) mitogenic effects on the uterus. To test these hypotheses, we compared E2's mitogenic effects on the uteri of Igf1-targeted gene deletion (null) and wild-type littermate mice. The proportion of uterine cells involved in the cell cycle and G1- and S-phase kinetics were not significantly different in wild-type and Igf1-null mice. However, the appearance of E2-induced mitotic figures and cell number increases were profoundly retarded in Igf1-null uterine tissue. There was a significant increase in nuclear DNA concentration in Igf1-null cells, consistent with a G2 arrest. Interestingly, apoptotic cells were also significantly reduced in abundance, and the normal massive apoptotic response to E2 withdrawal was absent in the Igf1-null uterus. These data show that Igf1 is an essential mediator of E2's mitogenic effects, with a critical role not in G1 progression but in G2 progression.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-1311209, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-1739135, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-2171908, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-2202516, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-2226350, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-2683075, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-2718698, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-278988, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-312500, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-4335548, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-6697957, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-7527408, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-7564264, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-7684390, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-8137728, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-8196606, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-8276243, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-8496300, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-8626866, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-8813730, http://linkedlifedata.com/resource/pubmed/commentcorrection/10077676-9013342
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3287-91
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Insulin-like growth factor 1 is required for G2 progression in the estradiol-induced mitotic cycle.
pubmed:affiliation
Developmental Endocrinology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892, USA.
pubmed:publicationType
Journal Article, Comparative Study