pubmed-article:10074513 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C0319975 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C0007623 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C1550101 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
pubmed-article:10074513 | lifeskim:mentions | umls-concept:C0065678 | lld:lifeskim |
pubmed-article:10074513 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:10074513 | pubmed:dateCreated | 1999-4-19 | lld:pubmed |
pubmed-article:10074513 | pubmed:abstractText | Mannoproteins are important and abundant structural components of fungal cell walls. The MP1 gene encodes a cell wall mannoprotein of the pathogenic fungus Penicillium marneffei. In the present study, we show that Mp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Mp1p was capable of detecting this protein from the cell culture supernatant of P. marneffei at 10(4) cells/ml. The anti-Mp1p antibody is specific since it fails to react with any protein-form lysates of Candida albicans, Histoplasma capsulatum, or Cryptococcus neoformans by Western blotting. In addition, this Mp1p antigen-based ELISA is also specific for P. marneffei since the cell culture supernatants of the other three fungi gave negative results. Finally, a clinical evaluation of sera from penicilliosis patients indicates that 17 of 26 (65%) patients are Mp1p antigen test positive. Furthermore, a Mp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for P. marneffei carry a sensitive of 88% (23 of 26), with a positive predictive value of 100% and a negative predictive value of 96%. The specificities of the tests are high since none of the 85 control sera was positive by either test. | lld:pubmed |
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pubmed-article:10074513 | pubmed:language | eng | lld:pubmed |
pubmed-article:10074513 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10074513 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:10074513 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:10074513 | pubmed:month | Apr | lld:pubmed |
pubmed-article:10074513 | pubmed:issn | 0095-1137 | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:LeeCC | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:FULDMM | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:ChenDD | lld:pubmed |
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pubmed-article:10074513 | pubmed:author | pubmed-author:CauJJ | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:YuenK YKY | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:ChaiC LCL | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:TsangD NDN | lld:pubmed |
pubmed-article:10074513 | pubmed:author | pubmed-author:VanittanakomN... | lld:pubmed |
pubmed-article:10074513 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:10074513 | pubmed:volume | 37 | lld:pubmed |
pubmed-article:10074513 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:10074513 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:10074513 | pubmed:pagination | 981-6 | lld:pubmed |
pubmed-article:10074513 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
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pubmed-article:10074513 | pubmed:year | 1999 | lld:pubmed |
pubmed-article:10074513 | pubmed:articleTitle | Detection of cell wall mannoprotein Mp1p in culture supernatants of Penicillium marneffei and in sera of penicilliosis patients. | lld:pubmed |
pubmed-article:10074513 | pubmed:affiliation | Department of Microbiology, The University of Hong Kong. lcao@hkucc.Hku.hk | lld:pubmed |
pubmed-article:10074513 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:10074513 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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