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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-4-19
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pubmed:abstractText |
Mannoproteins are important and abundant structural components of fungal cell walls. The MP1 gene encodes a cell wall mannoprotein of the pathogenic fungus Penicillium marneffei. In the present study, we show that Mp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Mp1p was capable of detecting this protein from the cell culture supernatant of P. marneffei at 10(4) cells/ml. The anti-Mp1p antibody is specific since it fails to react with any protein-form lysates of Candida albicans, Histoplasma capsulatum, or Cryptococcus neoformans by Western blotting. In addition, this Mp1p antigen-based ELISA is also specific for P. marneffei since the cell culture supernatants of the other three fungi gave negative results. Finally, a clinical evaluation of sera from penicilliosis patients indicates that 17 of 26 (65%) patients are Mp1p antigen test positive. Furthermore, a Mp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for P. marneffei carry a sensitive of 88% (23 of 26), with a positive predictive value of 100% and a negative predictive value of 96%. The specificities of the tests are high since none of the 85 control sera was positive by either test.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-16593749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-3293165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-6322947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-7574493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-7665670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-7714217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-7768807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-7912350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-7914566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-8308105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-8816141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-8880509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-9157151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-9195077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-9388561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-9434352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-9488383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10074513-9738061
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
981-6
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:10074513-Antibodies, Fungal,
pubmed-meshheading:10074513-Antigens, Fungal,
pubmed-meshheading:10074513-Blotting, Western,
pubmed-meshheading:10074513-Cell Wall,
pubmed-meshheading:10074513-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10074513-Fungal Proteins,
pubmed-meshheading:10074513-Genes, Fungal,
pubmed-meshheading:10074513-Humans,
pubmed-meshheading:10074513-Membrane Glycoproteins,
pubmed-meshheading:10074513-Mycoses,
pubmed-meshheading:10074513-Penicillium,
pubmed-meshheading:10074513-Predictive Value of Tests
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pubmed:year |
1999
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pubmed:articleTitle |
Detection of cell wall mannoprotein Mp1p in culture supernatants of Penicillium marneffei and in sera of penicilliosis patients.
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pubmed:affiliation |
Department of Microbiology, The University of Hong Kong. lcao@hkucc.Hku.hk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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