Linked Life Data

10025505

Source:http://linkedlifedata.com/resource/pubmed/id/10025505

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-2-25
pubmed:databankReference
pubmed:abstractText
The potential use of prostate secretory protein of 94 amino acids (PSP94) as a diagnostic biomarker or a therapeutic agent for prostate cancer has been reported. In order to establish an animal model to further elucidate on its biological role, we cloned the mouse PSP94 cDNA (approximately 500 bp) by reverse transcriptase-polymerase chain reaction (RT-PCR) and disclosed its genomic structure. The whole mouse PSP94 gene (approximately 23 kb) was amplified by long and accurate-PCR and also cloned by screening of a mouse embryo stem-cell genomic library. Computational and statistical analyses have demonstrated several highly conserved characteristics of PSP94 among different species. Comparison of PSP94 from human, two primates, pig, and rodents revealed that the most significant feature is that PSP94 is rich in cysteines (10% of the total sequence) and their positions are highly conserved. The three intron-four exon structure of the human PSP94 gene and the consensus sequence (....GT-intron-AG...) for mRNA splicing are also strongly conserved. A high divergence in cDNA sequence in the protein-coding region and also in the genomic sequence of PSP94 was also observed among these species. Comparing with alpha-globin, a typical evolutionally conserved gene, with the PSP94 gene, the rate of nonsynonymous changes per site per year (kN) is 2 to 6 times higher, indicating that PSP94 gene has been under far fewer evolutionary constraints than other genes and has a potential role as a species barrier in reproductive biology. In order to test this hypothesis, we investigated the gene expression of PSP94 and its tissue distribution in various rodent tissues by RT-PCR and in situ hybridization (ISH). Gene expression was found only in the prostate, suggesting that PSP94 is probably more tissue specific in the prostate of rodents than in mammals. The ISH analysis also revealed a prostate lobe-specific expression of the PSP94 gene in both mice and rats. It was strongly expressed in the lateral prostate, but the findings were negative in the dorsal and ventral lobe. Therefore, it is hypothesized that one of the primary functions of rodent PSP94, as a major prostate secretory protein, is related to reproductive biology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10025505-Amino Acid Sequence, pubmed-meshheading:10025505-Animals, pubmed-meshheading:10025505-Base Sequence, pubmed-meshheading:10025505-Cell Line, pubmed-meshheading:10025505-Cloning, Molecular, pubmed-meshheading:10025505-Cysteine, pubmed-meshheading:10025505-DNA, Complementary, pubmed-meshheading:10025505-Evolution, Molecular, pubmed-meshheading:10025505-Gene Expression, pubmed-meshheading:10025505-Genome, pubmed-meshheading:10025505-Humans, pubmed-meshheading:10025505-In Situ Hybridization, pubmed-meshheading:10025505-Male, pubmed-meshheading:10025505-Mice, pubmed-meshheading:10025505-Molecular Sequence Data, pubmed-meshheading:10025505-Organ Specificity, pubmed-meshheading:10025505-Peptides, pubmed-meshheading:10025505-Prostate, pubmed-meshheading:10025505-Prostatic Secretory Proteins, pubmed-meshheading:10025505-Proteins, pubmed-meshheading:10025505-Rats, pubmed-meshheading:10025505-Rats, Sprague-Dawley, pubmed-meshheading:10025505-Seminal Plasma Proteins, pubmed-meshheading:10025505-Sequence Alignment, pubmed-meshheading:10025505-Sequence Analysis, DNA
pubmed:year
1999
pubmed:articleTitle
cDNA, genomic cloning, and gene expression analysis of mouse PSP94 (prostate secretory protein of 94 amino acids).
pubmed:affiliation
Department of Surgery, University of Western Ontario, London, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't