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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0052181,
umls-concept:C0052191,
umls-concept:C0086222,
umls-concept:C0205195,
umls-concept:C0205227,
umls-concept:C0205410,
umls-concept:C0332255,
umls-concept:C0439851,
umls-concept:C0449774,
umls-concept:C1552596,
umls-concept:C1705733,
umls-concept:C1947931,
umls-concept:C2348205
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pubmed:issue |
8
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pubmed:dateCreated |
1999-3-18
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pubmed:abstractText |
Spatial gene expression in the intestine is mediated by specific regulatory sequences. The three genes of the apoA-I/C-III/A-IV cluster are expressed in the intestine following cephalocaudal and crypt-to-villus axes. Previous studies have shown that the -780/-520 enhancer region of the apoC-III gene directs the expression of the apoA-I gene in both small intestinal villi and crypts, implying that other unidentified elements are necessary for a normal intestinal pattern of apoA-I gene expression. In this study, we have characterized transgenic mice expressing the chloramphenicol acetyltransferase gene under the control of different regions of the apoC-III and apoA-IV promoters. We found that the -890/+24 apoC-III promoter directed the expression of the reporter gene in crypts and villi and did not follow a cephalocaudal gradient of expression. In contrast, the -700/+10 apoA-IV promoter linked to the -500/-890 apoC-III enhancer directed the expression of the reporter gene in enterocytes with a pattern of expression similar to that of the endogenous apoA-IV gene. Furthermore, linkage of the -700/-310 apoA-IV distal promoter region to the -890/+24 apoC-III promoter was sufficient to restore the appropriate pattern of intestinal expression of the reporter gene. These findings demonstrate that the -700/-310 distal region of the apoA-IV promoter contains regulatory elements that, in combination with proximal promoter elements and the -500/-890 enhancer, are necessary and sufficient to restrict apoC-III and apoA-IV gene expression to villus enterocytes of the small intestine along the cephalocaudal axis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein C-III,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins A,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins C,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/apolipoprotein A-IV
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:CardonJJ,
pubmed-author:ChambazJJ,
pubmed-author:ChateletF PFP,
pubmed-author:ChauffetonVV,
pubmed-author:Cywiner-GolenzerCC,
pubmed-author:JanvierP LPL,
pubmed-author:KalopissisA DAD,
pubmed-author:Le BeyecJJ,
pubmed-author:MaoJ XJX,
pubmed-author:Pinçon-RaymondMM,
pubmed-author:ZannisVV
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4954-61
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9988739-Animals,
pubmed-meshheading:9988739-Apolipoprotein C-III,
pubmed-meshheading:9988739-Apolipoproteins A,
pubmed-meshheading:9988739-Apolipoproteins C,
pubmed-meshheading:9988739-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:9988739-Enhancer Elements, Genetic,
pubmed-meshheading:9988739-Gene Expression Regulation,
pubmed-meshheading:9988739-Intestine, Small,
pubmed-meshheading:9988739-Mice,
pubmed-meshheading:9988739-Mice, Transgenic,
pubmed-meshheading:9988739-RNA, Messenger,
pubmed-meshheading:9988739-Regulatory Sequences, Nucleic Acid
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pubmed:year |
1999
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pubmed:articleTitle |
The -700/-310 fragment of the apolipoprotein A-IV gene combined with the -890/-500 apolipoprotein C-III enhancer is sufficient to direct a pattern of gene expression similar to that for the endogenous apolipoprotein A-IV gene.
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pubmed:affiliation |
U.505 INSERM and UPRESA CNRS 7079, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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