Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-3-16
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071128, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071129, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071130, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071131, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071132, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071133, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071134, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071135, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071136, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071137, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071138, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071139, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071140, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071141, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF071142, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF140676, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF140677
pubmed:abstractText
Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. Type strains of 10 RGM species were first studied. Each species had a unique nucleotide sequence, distinguishing it clearly from the other species. A panel of strains from the four main RGM species responsible for human infections, Mycobacterium abscessus, Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium peregrinum, was also studied. There were few sequence differences within each of these species (<2% of bases were different from the type strain sequence), and they had no effect on species assignment. hsp65 sequencing unambiguously differentiated M. chelonae and M. abscessus, two species difficult to identify by classical methods and 16S rRNA gene sequencing. The devised procedure is a rapid and reliable tool for the identification of RGM species.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-1280641, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-1352786, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-1581184, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-2507865, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-3029018, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7505291, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7698114, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7698122, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7699032, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7848059, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7852575, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-7907223, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8253968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8381805, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8396586, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8513048, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8789004, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8940463, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-8968884, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9041432, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9103630, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9276419, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9278615, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9350770, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9431937, http://linkedlifedata.com/resource/pubmed/commentcorrection/9986875-9431938
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
852-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
hsp65 sequencing for identification of rapidly growing mycobacteria.
pubmed:affiliation
Service de Microbiologie, Hôpital Necker-Enfants Malades, 75015 Paris, France.
pubmed:publicationType
Journal Article