Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1999-4-13
|
| pubmed:abstractText |
CD40 ligand (CD40L) gene-disrupted (CD40L-/-) mice were employed to examine the role of costimulatory signals via CD40L-CD40 interactions in mucosally induced tolerance. CD40L-/- and control (CD40L+/+) mice of the same C57BL/6 x 129/J background were immunized orally with 25 mg of OVA before systemic challenge with OVA in CFA. While CD40L+/+ mice showed reductions in Ag-specific T cell responses including delayed-type hypersensitivity (DTH) and proliferative responses, CD40L-/- mice underwent normal T cell responses. Further, cytokine analysis of splenic CD4+ T cells showed that both Th1-type (e.g., IFN-gamma and IL-2) and Th2-type (e.g., IL-4, IL-5, IL-6, and IL-10) responses were maintained in CD40L-/- mice orally immunized with OVA, whereas these cytokine responses in CD40L+/+ mice were significantly reduced. In addition, splenic CD4+ T cells from CD40L-/- mice orally immunized with OVA provided B cell help in Ag-specific Ab-forming cells when the cells were cultured with naive B cells in the presence of Ag and CD40L-transfected cell lines. In contrast, an identical culture condition containing splenic CD4+ T cells from orally tolerized CD40L+/+ mice did not exhibit helper activity. Taken together, these findings indicate that CD40L and CD40 interactions are essential for the induction of systemic T cell unresponsiveness to orally administered Ag.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1904-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9973457-Administration, Oral,
pubmed-meshheading:9973457-Animals,
pubmed-meshheading:9973457-Antigens, CD40,
pubmed-meshheading:9973457-B-Lymphocytes,
pubmed-meshheading:9973457-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9973457-CD40 Ligand,
pubmed-meshheading:9973457-Cytokines,
pubmed-meshheading:9973457-Dose-Response Relationship, Immunologic,
pubmed-meshheading:9973457-Down-Regulation,
pubmed-meshheading:9973457-Immune Tolerance,
pubmed-meshheading:9973457-Ligands,
pubmed-meshheading:9973457-Lymphocyte Cooperation,
pubmed-meshheading:9973457-Membrane Glycoproteins,
pubmed-meshheading:9973457-Mice,
pubmed-meshheading:9973457-Mice, Inbred C57BL,
pubmed-meshheading:9973457-Mice, Inbred Strains,
pubmed-meshheading:9973457-Mice, Knockout,
pubmed-meshheading:9973457-Mouth Mucosa,
pubmed-meshheading:9973457-Ovalbumin,
pubmed-meshheading:9973457-T-Lymphocyte Subsets,
pubmed-meshheading:9973457-Th1 Cells,
pubmed-meshheading:9973457-Th2 Cells,
pubmed-meshheading:9973457-Transfection
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Mucosally induced systemic T cell unresponsiveness to ovalbumin requires CD40 ligand-CD40 interactions.
|
| pubmed:affiliation |
Department of Oral Biology, Immunobiology Vaccine Center, University of Alabama Medical Center, Birmingham 35294, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|