Linked Life Data

9973456

Source:http://linkedlifedata.com/resource/pubmed/id/9973456

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-4-13
pubmed:abstractText
TCR gamma delta+ cells are enriched in the intestine mucosa and constitute approximately half of the intestinal intraepithelial lymphocytes (iIEL) in mice. They are likely activated by self and foreign Ags in situ, but little is known about how the activated gamma delta iIEL are regulated. In the iIEL compartment, IL-2 is produced by activated TCR alpha beta+ iIEL, and IL-15 message is detected in iIEL and in the epithelial cells. We found surface expression of IL-2 as well as IL-15Rs on activated gamma delta iIEL, and examined the effects of IL-2 and IL-15 on the survival and death of gamma delta iIEL during secondary stimulation through TCR. We found that both cytokines supported growth of the restimulated gamma delta iIEL, but exerted different effects on their survival. A significant higher number of live cells were recovered from the gamma delta iIEL cultures restimulated in IL-15 than in IL-2. Quantitation of apoptotic cells showed more cell death in the IL-2 group than in the IL-15 group. The cell death was associated with restimulation through TCR and was not caused by insufficient growth factor, thus representing activation-induced cell death. Western blot analyses found no difference in the levels of Bcl-2 and Bax proteins between the two groups. However, the level of Bcl-xL protein diminished with time in the IL-2 group whereas the level was sustained in the IL-15 group, which may contribute to the pro-survival effect of IL-15. These results demonstrated that the survival of activated gamma delta iIEL is differentially regulated by IL-2 and IL-15.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1896-903
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9973456-Animals, pubmed-meshheading:9973456-Apoptosis, pubmed-meshheading:9973456-Cell Division, pubmed-meshheading:9973456-Cell Survival, pubmed-meshheading:9973456-Cells, Cultured, pubmed-meshheading:9973456-Dose-Response Relationship, Immunologic, pubmed-meshheading:9973456-Interleukin-15, pubmed-meshheading:9973456-Interleukin-2, pubmed-meshheading:9973456-Intestinal Mucosa, pubmed-meshheading:9973456-Lymphocyte Activation, pubmed-meshheading:9973456-Male, pubmed-meshheading:9973456-Mice, pubmed-meshheading:9973456-Mice, Inbred C57BL, pubmed-meshheading:9973456-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:9973456-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:9973456-Receptors, Interleukin-15, pubmed-meshheading:9973456-Receptors, Interleukin-2, pubmed-meshheading:9973456-T-Lymphocyte Subsets, pubmed-meshheading:9973456-bcl-X Protein
pubmed:year
1999
pubmed:articleTitle
Differential effects of IL-2 and IL-15 on the death and survival of activated TCR gamma delta+ intestinal intraepithelial lymphocytes.
pubmed:affiliation
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't