Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1999-4-13
|
| pubmed:abstractText |
From the biobreeding-diabetic prone (BB-DP) rat, an animal model for endocrine autoimmunity, phenotype and function of splenic dendritic cells (DC) were studied. Furthermore, the suppressive effect of peritoneal macrophages (pMphi) from the BB-DP rat in the MLR was investigated. Lower numbers of splenic DC were isolated from BB-DP rats than from control Wistar rats. In the preautoimmune phase, DC of the BB-DP rat had a lower surface MHC class II expression (and in preliminary data, a lower CD80 expression), ingested more bacteria, and had a lower stimulatory potency in the syngeneic (syn)MLR as compared with control DC. During disease development, the MHC class II expression further decreased, and a low stimulatory activity became evident in the allogeneic (allo)MLR. With regard to the expansion of suppressor/regulatory T cells, a lower percentage of RT6+ T cells but higher percentages of CD45RClow T cells were induced by BB-DP DC in synMLR, but not in alloMLR. An increase in the CD4/CD8 T cell ratio was observed in both the syn- and alloMLR due to a relative weak expansion of CD8+ T cells with DC of the BB-DP rat. Resident pMphi isolated from BB-DP or Wistar rats were equally effective in suppressing the DC-driven synMLR. In conclusion, splenic DC from the BB-DP rat have a lower accessory cell function already at young age, before the development of disease, and expanded different subsets of effector/suppressor T cells in vitro as compared with those from Wistar rats. The dysfunction of DC from BB-DP rats is likely to be caused by their relative immaturity as indicated by their low class II and costimulatory molecule expression and relatively high phagocytic activity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dextrans,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/fluorescein isothiocyanate dextran
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1795-801
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9973444-Animals,
pubmed-meshheading:9973444-Autoimmune Diseases,
pubmed-meshheading:9973444-Cell Differentiation,
pubmed-meshheading:9973444-Dendritic Cells,
pubmed-meshheading:9973444-Dextrans,
pubmed-meshheading:9973444-Diabetes Mellitus, Type 1,
pubmed-meshheading:9973444-Escherichia coli,
pubmed-meshheading:9973444-Female,
pubmed-meshheading:9973444-Fluorescein-5-isothiocyanate,
pubmed-meshheading:9973444-Histocompatibility Antigens Class II,
pubmed-meshheading:9973444-Lymphocyte Activation,
pubmed-meshheading:9973444-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:9973444-Macrophages, Peritoneal,
pubmed-meshheading:9973444-Male,
pubmed-meshheading:9973444-Phagocytosis,
pubmed-meshheading:9973444-Phenotype,
pubmed-meshheading:9973444-Rats,
pubmed-meshheading:9973444-Rats, Inbred BB,
pubmed-meshheading:9973444-Rats, Wistar,
pubmed-meshheading:9973444-Spleen,
pubmed-meshheading:9973444-T-Lymphocyte Subsets
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Signs of immaturity of splenic dendritic cells from the autoimmune prone biobreeding rat: consequences for the in vitro expansion of regulator and effector T cells.
|
| pubmed:affiliation |
Department of Immunology, Erasmus University, Rotterdam, The Netherlands. delemarre@immu.fgg.eur.nl
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|