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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-4-13
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pubmed:abstractText |
The induction of clonal anergy in a T cell inhibits IL-2 secretion because of the development of a proximal signal transduction defect. Fusion of anergic murine T cells to human Jurkat T leukemia cells and formation of heterokaryons failed to result in a complementation of this signaling defect and restoration of murine IL-2 mRNA inducibility. Instead, signal transduction to the human IL-2 gene became disrupted. Heterokaryons formed by the fusion of anergic murine T cells to normal murine T cells also failed to accumulate intracellular IL-2 protein in response to stimulation either with the combination of CD3 and CD28 mAbs or with ionomycin plus a protein kinase C-activating phorbol ester. The results argue against a loss-of-function signaling defect as the sole basis for clonal anergy induction and document the presence of a dominant-acting repressor molecule that inhibits signal transduction to the IL-2 gene within viable anergic T cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1460-5
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9973402-Animals,
pubmed-meshheading:9973402-Base Sequence,
pubmed-meshheading:9973402-Cell Fusion,
pubmed-meshheading:9973402-Cell Line,
pubmed-meshheading:9973402-Clonal Anergy,
pubmed-meshheading:9973402-DNA Primers,
pubmed-meshheading:9973402-Gene Expression Regulation,
pubmed-meshheading:9973402-Genetic Complementation Test,
pubmed-meshheading:9973402-Humans,
pubmed-meshheading:9973402-Interleukin-2,
pubmed-meshheading:9973402-Ionomycin,
pubmed-meshheading:9973402-Jurkat Cells,
pubmed-meshheading:9973402-Lymphocyte Activation,
pubmed-meshheading:9973402-Mice,
pubmed-meshheading:9973402-Models, Biological,
pubmed-meshheading:9973402-Repressor Proteins,
pubmed-meshheading:9973402-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9973402-T-Lymphocytes,
pubmed-meshheading:9973402-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9973402-Transcriptional Activation
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pubmed:year |
1999
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pubmed:articleTitle |
Evidence for repression of IL-2 gene activation in anergic T cells.
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pubmed:affiliation |
Department of Medicine and Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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