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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-3-18
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pubmed:abstractText |
This study investigated the ability of membrane-damaged sperm to undergo sperm head decondensation and male pronuclear forrmation following intracytoplasmic sperm injection. For induction of membrane-damage, sperm were exposed to 0.1% Triton X-100 for a few seconds. The single sperm curling (SSC) test was performed to evaluate the viability of the sperm. Of the injected oocytes 49 and 47% developed male pronuclei in the experimental and control groups, respectively. Sperm head decondensation was much higher in the membrane-damaged group (84 versus 70%). This study clearly shows that ICSI of membrane-damaged sperm does not affect pronuclear formation, although sperm head decondensation is much higher.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0148-5016
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9973139-Animals,
pubmed-meshheading:9973139-Cell Membrane,
pubmed-meshheading:9973139-Cell Nucleus,
pubmed-meshheading:9973139-Cell Survival,
pubmed-meshheading:9973139-Cricetinae,
pubmed-meshheading:9973139-Female,
pubmed-meshheading:9973139-Fertilization in Vitro,
pubmed-meshheading:9973139-Humans,
pubmed-meshheading:9973139-Male,
pubmed-meshheading:9973139-Microinjections,
pubmed-meshheading:9973139-Sperm Head,
pubmed-meshheading:9973139-Spermatozoa
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pubmed:articleTitle |
Influence of sperm plasma membrane destruction on human sperm head decondensation and pronuclear formation.
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pubmed:affiliation |
Department of Obstetrics and Gynaecology, National University of Singapore, Singapore.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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