Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-3-4
pubmed:abstractText
In order to determine the parameters that govern the activity of a ribozyme in vivo, we made a systematic analysis of chimeric tRNAVal ribozymes by measuring their cleavage activities in vitro as well as the steady-state levels of transcripts, the half-lives of transcribed tRNAVal ribozymes, and their activities in both HeLa and H9 cells. These analyses were conducted by the use of transient expression systems in HeLa cells and stable transformants that express ribozymes. Localization of transcripts appeared to be determined by the higher-order structure of each transcribed tRNAVal ribozyme. Since colocalization of the ribozyme with its target RNA is important for strong activity of the ribozyme in vivo, the best system for tRNA-based expression seems to be one in which the structure of the transcript is different from that of the natural tRNA precursor so that processing of the tRNAVal ribozyme can be avoided. At the same time, the structure of the transcript must be similar enough to allow recognition, probably by an export receptor, so that the transcript can be exported to the cytoplasm to ensure colocalization with its target. In the case of several tRNAVal ribozymes that we constructed, inspection of computer-predicted secondary structures enabled us to control the export of transcripts. We found that only a ribozyme that was transcribed at a high level and that had a sufficiently long half-life, within cells, had significant activity when used to withstand a challenge by human immunodeficiency virus type 1.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-1366622, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-1716459, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-1885765, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2107573, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2241948, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2247070, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2441261, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2446865, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2457170, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2684648, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-2822260, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-3016298, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-3023191, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-6197186, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-6297745, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-6320115, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-6328526, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-6912791, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7489499, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7510389, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7544984, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7584058, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7597097, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7610054, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7864879, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-7975207, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8157667, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8241158, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8248243, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8248806, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8327516, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8413588, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8657127, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8666273, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8702616, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8760887, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8855316, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-8990401, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9012792, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9055033, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9068795, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9122187, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9174101, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9405614, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9435228, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9607768, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9708893, http://linkedlifedata.com/resource/pubmed/commentcorrection/9971765-9788354
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1868-77
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Factors governing the activity in vivo of ribozymes transcribed by RNA polymerase III.
pubmed:affiliation
National Institute for Advanced Interdisciplinary Research, AIST, MITI, Tsukuba Science City 305-8562, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8539, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't