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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2 Pt 1
|
| pubmed:dateCreated |
1999-3-30
|
| pubmed:abstractText |
The role of adenosine A1 receptors (A1R) in reflex-evoked short-circuit current (Isc) indicative of chloride secretion was studied in the guinea pig colon. The A1R antagonist 8-cyclopentyltheophylline (CPT) enhanced reflex-evoked Isc. Adenosine deaminase and the nucleoside transport inhibitor S-(4-nitrobenzyl)-6-thioinosine enhanced and reduced reflex-induced Isc, respectively. The A1R agonist 2-chloro-N6-cyclopentyladenosine (CCPA) inhibited reflex-evoked Isc at nanomolar concentrations, and its action was antagonized by CPT. In the presence of either N-acetyl-5-hydroxytryptophyl-5-hydroxytryptophan amide to block the 5-hydroxytryptamine (5-HT)-mediated pathway or piroxicam to block the prostaglandin-mediated pathway, CCPA reduced the residual reflex-evoked Isc. CCPA reduced the response to a 5-HT pulse without affecting the tetrodotoxin-insensitive Isc responses to carbachol or forskolin. Immunoreactivity for A1R was detected in the membrane (10% of neurons) and cytoplasm (90% of neurons) of neural protein gene product 9.5-immunoreactive (or S-100-negative) submucosal neurons, in glia, and in the muscularis mucosa. A1R immunoreactivity in a majority of neurons remained elevated in the cytoplasm despite preincubation with adenosine deaminase or CPT. A1R immunoreactivity colocalized in synaptophysin-immunoreactive presynaptic varicose nerve terminals. The results indicate that endogenous adenosine binding to high-affinity A1R on submucosal neurons acts as a physiological brake to suppress reflex-evoked Isc indicative of chloride secretion.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P1 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P1 Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P1,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thiolester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin Thiolesterase
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0002-9513
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
276
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
G451-62
|
| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:9950819-Adenosine,
pubmed-meshheading:9950819-Animals,
pubmed-meshheading:9950819-Colon,
pubmed-meshheading:9950819-Electric Conductivity,
pubmed-meshheading:9950819-Fluorescent Antibody Technique,
pubmed-meshheading:9950819-Ganglia,
pubmed-meshheading:9950819-Guinea Pigs,
pubmed-meshheading:9950819-Intestinal Mucosa,
pubmed-meshheading:9950819-Male,
pubmed-meshheading:9950819-Neurons,
pubmed-meshheading:9950819-Purinergic P1 Receptor Agonists,
pubmed-meshheading:9950819-Purinergic P1 Receptor Antagonists,
pubmed-meshheading:9950819-Receptors, Purinergic P1,
pubmed-meshheading:9950819-Reflex,
pubmed-meshheading:9950819-S100 Proteins,
pubmed-meshheading:9950819-Submucous Plexus,
pubmed-meshheading:9950819-Thiolester Hydrolases,
pubmed-meshheading:9950819-Tissue Distribution,
pubmed-meshheading:9950819-Ubiquitin Thiolesterase
|
| pubmed:year |
1999
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| pubmed:articleTitle |
Activation of neuronal adenosine A1 receptors suppresses secretory reflexes in the guinea pig colon.
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| pubmed:affiliation |
Department of Pharmacology, The Ohio State University, Columbus, Ohio 43210, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|