Linked Life Data

9933970

Source:http://linkedlifedata.com/resource/pubmed/id/9933970

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11-12
pubmed:dateCreated
1999-2-26
pubmed:abstractText
The presence of (Na(+)+K+)ATPase activity in CL14 clone and NIH NTY strain of Trypanosoma cruzi epimastigotes is demonstrated. A Na+ plus K+ stimulated ATPase activity is found in both strains. The optimal Na+/K+ ratio is 5:1 and 9:1 in CL14 clone and NIH NTY strain, respectively. In both strains, vanadate completely inhibits the ouabain-sensitive ATPase activity indicating that it belongs to the P-type (E1/E2) family of ion-transporting ATPases. The I50 for vanadate is 0.66 +/- 0.04 and 0.04 +/- 0.02 microM in CL14 clone and NIH NTY strain, respectively. These data indicate that both strains of T. cruzi epimastigotes express the ouabain- and vanadate-sensitive (Na(+)+K+)ATPase activity. On the other hand, the discrepancy between the parameters analyzed for the inhibitors suggests that they express different isoforms of this enzyme.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0939-5075
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1049-54
pubmed:dateRevised
2009-11-4
pubmed:meshHeading
pubmed:articleTitle
Trypanosoma cruzi epimastigotes express the ouabain- and vanadate-sensitive (Na(+)+K+)ATPase activity.
pubmed:affiliation
Laboratório de Fisiologia Renal, Universidade Federal do Rio de Janeiro, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't