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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1999-3-23
|
| pubmed:abstractText |
We studied the effects of 8 weeks of streptozotocin (STZ)-induced diabetes on the density and the pharmacological properties of alpha1-adrenoceptors in the rat prostate using receptor-binding experiments with [125I]iodo-2[beta-(4-hydroxyphenyl)-ethylaminomethyl]tetralone [125I]HEAT. Saturation experiments showed the presence of specific [125I]HEAT-binding sites in the control and diabetic rat prostate and that the induction of diabetes significantly decreased the density of [125I]HEAT-binding sites in the rat prostate. [125I]HEAT-binding sites in the prostate of both groups were inhibited by prazosin (nonselective), spiperone (alpha1B-selective), WB4101 and 5-methylurapidil (alpha1A-selective) and BMY7378 (alpha1D-selective) with the following rank order of Ki values: prazosin < WB4101 < 5-methylurapidil < spiperone < BMY7378, indicating a similar pharmacological profile of alpha1-adrenoceptor in the 2 groups. Comparing the Ki values of the rat prostate with those obtained from the rat submaxillary gland (alpha1A), rat spleen (alpha1B), rat vas deferens (alpha1A + alpha1B) and those reported for cloned alpha1D, indicates the predominance of the alpha1A + alpha1B or the alpha1A subtype in the rat prostate. The present study demonstrates that STZ-induced diabetes downregulates the expression of alpha1-adrenoceptor in the rat prostate, without significantly affecting the receptor subtype specificity.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0042-1138
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
61
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
147-53
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9933834-Adrenergic alpha-Antagonists,
pubmed-meshheading:9933834-Analysis of Variance,
pubmed-meshheading:9933834-Animals,
pubmed-meshheading:9933834-Binding Sites,
pubmed-meshheading:9933834-Culture Techniques,
pubmed-meshheading:9933834-Diabetes Mellitus, Experimental,
pubmed-meshheading:9933834-Disease Models, Animal,
pubmed-meshheading:9933834-Down-Regulation,
pubmed-meshheading:9933834-Linear Models,
pubmed-meshheading:9933834-Male,
pubmed-meshheading:9933834-Prostate,
pubmed-meshheading:9933834-Rats,
pubmed-meshheading:9933834-Rats, Sprague-Dawley,
pubmed-meshheading:9933834-Receptors, Adrenergic, alpha,
pubmed-meshheading:9933834-Reference Values,
pubmed-meshheading:9933834-Spleen,
pubmed-meshheading:9933834-Streptozocin,
pubmed-meshheading:9933834-Submandibular Gland,
pubmed-meshheading:9933834-Vas Deferens
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Properties of alpha-1-adrenergic receptors in the rat prostate: effect of experimental diabetes.
|
| pubmed:affiliation |
Section of Urology, Yale University School of Medicine, New Haven, Conn. 06520, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|