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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-2-16
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pubmed:abstractText |
Dendritic cells (DC) expanded in the presence of GM-CSF from the bone marrow of C57BL/6 mice process Gram-negative bacteria expressing the model antigen Crl-OVA for peptide presentation on MHC class I molecules. Here we show that presentation of OVA(257-264) processed by DC co-incubated with E. coli expressing Crl-OVA, which contains the Kb-binding OVA(257-264) epitope, occurs by a cytosolic MHC-I presentation pathway. First, we demonstrate the requirement for the transporter associated with antigen processing (TAP) by showing that DC from TAP1-/- mice co-incubated with E. coli expressing Crl-OVA did not result in Kb presentation of OVA(257-264). Second, the proteasome inhibitor MG132 abrogated presentation of OVA(257-264) on Kb when C57BL/6 DC phagocytosed and processed E. coli expressing Crl-OVA. Third, inhibiting protein synthesis using cycloheximide or blocking exocytosis of newly synthesized proteins from the endoplasmic reticulum using brefeldin A abrogated presentation of OVA(257-264) processed from bacteria expressing Crl-OVA by C57BL/6 DC. Finally, peptide regurgitation and loading of OVA(257-264) on neighboring bystander Kb-expressing antigen-presenting cells after BALB/c (H-2d) DC phagocytosed E. coli expressing Crl-OVA could not be detected. Together, these data support a cytosolic MHC-I presentation pathway for OVA(257-264) processed from E. coli expressing Crl-OVA by bone marrow-derived DC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Crl protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tap1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/csgA protein, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
180-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9933099-ATP-Binding Cassette Transporters,
pubmed-meshheading:9933099-Animals,
pubmed-meshheading:9933099-Antigen Presentation,
pubmed-meshheading:9933099-Antigens, Bacterial,
pubmed-meshheading:9933099-Bacterial Proteins,
pubmed-meshheading:9933099-Dendritic Cells,
pubmed-meshheading:9933099-Escherichia coli,
pubmed-meshheading:9933099-Escherichia coli Proteins,
pubmed-meshheading:9933099-Histocompatibility Antigens Class I,
pubmed-meshheading:9933099-Macrophages,
pubmed-meshheading:9933099-Mice,
pubmed-meshheading:9933099-Mice, Inbred BALB C,
pubmed-meshheading:9933099-Mice, Inbred C57BL,
pubmed-meshheading:9933099-Mice, Knockout,
pubmed-meshheading:9933099-Ovalbumin,
pubmed-meshheading:9933099-Phagocytosis,
pubmed-meshheading:9933099-Recombinant Fusion Proteins,
pubmed-meshheading:9933099-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
Classical MHC class I peptide presentation of a bacterial fusion protein by bone marrow-derived dendritic cells.
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pubmed:affiliation |
Department of Cell and Molecular Biology, Lund University, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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