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rdf:type |
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lifeskim:mentions |
umls-concept:C0001511,
umls-concept:C0007595,
umls-concept:C0010453,
umls-concept:C0016055,
umls-concept:C0024623,
umls-concept:C0025932,
umls-concept:C0041243,
umls-concept:C0055023,
umls-concept:C0086418,
umls-concept:C0334227,
umls-concept:C1514559,
umls-concept:C1519697,
umls-concept:C1948023
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pubmed:issue |
7
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pubmed:dateCreated |
1999-2-25
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pubmed:abstractText |
It has previously been reported that the trypsinogen gene is expressed in various human cancers. To investigate the possible role of trypsin in tumor malignancy, trypsinogen-1 cDNA was introduced into the human gastric carcinoma cell line MKN-1. The overexpression of trypsinogen-1 in MKN-1 cells stimulated cellular growth and adhesion to fibronectin and vitronectin when the trypsinogen activator enterokinase was added into the culture. Enterokinase treatment of the conditioned medium of the MKN-1 transfectants partially converted the proforms of gelatinases B and A to their apparent active forms. When the MKN-1 transfectants expressing trypsinogen-1 were intraperitoneally transplanted into nude mice, the mice frequently produced tumors in the colon, spleen and liver. However, the mice implanted with control MKN-1 cells produced no tumors. These results strongly suggest that tumor-derived trypsin contributes to the disseminated growth of some types of cancer cells including gastric cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Enteropeptidase,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Gelatinases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsinogen,
http://linkedlifedata.com/resource/pubmed/chemical/Vitronectin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0262-0898
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
613-22
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9932608-Adenocarcinoma,
pubmed-meshheading:9932608-Animals,
pubmed-meshheading:9932608-Blotting, Northern,
pubmed-meshheading:9932608-Cell Adhesion,
pubmed-meshheading:9932608-Cell Division,
pubmed-meshheading:9932608-Collagenases,
pubmed-meshheading:9932608-DNA, Complementary,
pubmed-meshheading:9932608-Enteropeptidase,
pubmed-meshheading:9932608-Fibronectins,
pubmed-meshheading:9932608-Gelatinases,
pubmed-meshheading:9932608-Humans,
pubmed-meshheading:9932608-Immunoblotting,
pubmed-meshheading:9932608-Matrix Metalloproteinase 2,
pubmed-meshheading:9932608-Matrix Metalloproteinase 9,
pubmed-meshheading:9932608-Metalloendopeptidases,
pubmed-meshheading:9932608-Mice,
pubmed-meshheading:9932608-Mice, Nude,
pubmed-meshheading:9932608-Neoplasm Transplantation,
pubmed-meshheading:9932608-Serine Endopeptidases,
pubmed-meshheading:9932608-Stomach Neoplasms,
pubmed-meshheading:9932608-Transfection,
pubmed-meshheading:9932608-Trypsin,
pubmed-meshheading:9932608-Trypsinogen,
pubmed-meshheading:9932608-Tumor Cells, Cultured,
pubmed-meshheading:9932608-Vitronectin
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pubmed:year |
1998
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pubmed:articleTitle |
Stimulation of cellular growth and adhesion to fibronectin and vitronectin in culture and tumorigenicity in nude mice by overexpression of trypsinogen in human gastric cancer cells.
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pubmed:affiliation |
Kihara Institute for Biological Research, and Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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