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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-3-16
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pubmed:databankReference | |
pubmed:abstractText |
We have isolated and sequenced cDNA clones encoding a 90-kDa heat shock protein (HSP90) from a porcine brain cDNA library. The sequence of the 2202-nucleotide coding region showed 88.6% homology with that of the human homologue. Moreover, the deduced amino acid sequence of the porcine hsp90 cDNA was 99.7% identical to that of the human counterpart, with a difference of only three amino acids in a total of 733 residues. Expression of the gene was greatly increased in cultured cells during recovery from heat shock treatment at 45 degrees C for 60 min. Three major transcripts 2.2, 3.0, and 4.1kb in size were detected by Northern blot hybridization. These transcripts were further identified in a whole-pig hyperthermia experiment. These three hsp90 transcripts were constitutively expressed in porcine tissues including kidney, liver, brain, and heart, and their levels were markedly enhanced during recovery from 30-min hyperthermia treatment at 43 degrees C. Furthermore, we found that HSP90 was preferentially expressed in pituitary gland, brain, adrenal gland, and testis, in comparison to the other tissues.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
226
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
307-15
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9931505-Amino Acid Sequence,
pubmed-meshheading:9931505-Animals,
pubmed-meshheading:9931505-Base Sequence,
pubmed-meshheading:9931505-Cardiomegaly,
pubmed-meshheading:9931505-Cells, Cultured,
pubmed-meshheading:9931505-Cloning, Molecular,
pubmed-meshheading:9931505-DNA, Complementary,
pubmed-meshheading:9931505-Death, Sudden, Cardiac,
pubmed-meshheading:9931505-Fever,
pubmed-meshheading:9931505-Germ-Free Life,
pubmed-meshheading:9931505-HSP90 Heat-Shock Proteins,
pubmed-meshheading:9931505-Humans,
pubmed-meshheading:9931505-Molecular Sequence Data,
pubmed-meshheading:9931505-Muscle, Smooth, Vascular,
pubmed-meshheading:9931505-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9931505-Sequence Homology, Amino Acid,
pubmed-meshheading:9931505-Swine
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pubmed:year |
1999
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pubmed:articleTitle |
Molecular cloning and characterization of porcine cDNA encoding a 90-kDa heat shock protein and its expression following hyperthermia.
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pubmed:affiliation |
Cardiovascular Research Center, Department of Comparative Medicine, Pig Research Institute, Taiwan, Chunan, Miaoli, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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