Linked Life Data

9931456

Source:http://linkedlifedata.com/resource/pubmed/id/9931456

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-2-23
pubmed:databankReference
pubmed:abstractText
Screening of cDNA libraries for the homologous vertebrate proteins high mobility group (HMG) 1 and 2 using DNA probes based on the coding sequences is likely to result in isolation of both HMG1 and HMG2 clones, as well as pseudogenes, which may be transcribed at low levels. However, the 3'-untranslated regions (UTRs) of HMG1 and 2 are quite distinct, and unusually conserved across species. We have used this property to select the true chicken HMG1 cDNA clone from a chicken lymphocyte cDNA library in lambdagt11, using a probe based on the 3'-UTR of rat HMG1 cDNA. The chicken HMG1 cDNA clone is very similar to all the complete HMG1 cDNA clones isolated so far. We suggest that the sequence designated chicken HMG1 in the GenBank Data Library (Accession number D14314) is, in fact, that of HMG2a [and moreover that the recently reported mouse clone (Accession number AF022465), proposed to encode a new HMG protein, HMG4, is also likely to encode an HMG2a, based on the translated amino-acid sequence and 3'-UTR]. We also report much improved expression of intact recombinant HMG1 in Escherichia coli by the use of chloramphenicol rather than ampicillin selection and conditions that limit cell growth. This should be general for all members of the HMG1 (and 2) family which may be toxic to cells (possibly because of the long acidic tail), and may also prove useful in the production of other such proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-105
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9931456-3' Untranslated Regions, pubmed-meshheading:9931456-Amino Acid Sequence, pubmed-meshheading:9931456-Animals, pubmed-meshheading:9931456-Base Sequence, pubmed-meshheading:9931456-Chickens, pubmed-meshheading:9931456-Cloning, Molecular, pubmed-meshheading:9931456-Codon, pubmed-meshheading:9931456-Conserved Sequence, pubmed-meshheading:9931456-DNA, Complementary, pubmed-meshheading:9931456-Escherichia coli, pubmed-meshheading:9931456-Gene Expression Regulation, Bacterial, pubmed-meshheading:9931456-High Mobility Group Proteins, pubmed-meshheading:9931456-Molecular Sequence Data, pubmed-meshheading:9931456-Recombinant Fusion Proteins, pubmed-meshheading:9931456-Sequence Alignment, pubmed-meshheading:9931456-Sequence Homology, Amino Acid, pubmed-meshheading:9931456-Sequence Homology, Nucleic Acid
pubmed:year
1998
pubmed:articleTitle
Selection of a cDNA clone for chicken high-mobility-group 1 (HMG1) protein through its unusually conserved 3'-untranslated region, and improved expression of recombinant HMG1 in Escherichia coli.
pubmed:affiliation
Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't