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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-2-11
|
| pubmed:abstractText |
Several lines of evidence support the role of oxidative stress, including increased lipid peroxidation, in the pathogenesis of Alzheimer's disease (AD). Lipid peroxidation generates various reactive aldehydes, such as 4-hydroxynonenal (HNE), which have been detected immunochemically in AD, particularly in neurofibrillary tangles, one of the major diagnostic lesions in AD brains. A recent study demonstrated that acrolein, the most reactive among the alpha,beta-unsaturated aldehyde products of lipid peroxidation, could be rapidly incorporated into proteins, generating a carbonyl derivative, a marker of oxidative stress to proteins. The current studies used an antibody raised against acrolein-modified keyhole limpet hemocyanin (KLH) to test whether acrolein modification of proteins occurs in AD. Double immunofluorescence revealed strong acrolein-KLH immunoreactivity in more than half of all paired helical filament (PHF)-1-labeled neurofibrillary tangles in AD cases. Acrolein-KLH immunoreactivity was also evident in a few neurons lacking PHF-1-positive neurofibrillary tangles. Light acrolein-KLH immunoreactivity occurred in dystrophic neurites surrounding the amyloid-beta core, which itself lacked acrolein-KLH staining. The pattern of acrolein-KLH immunostaining was similar to that of HNE. Control brains did not contain any acrolein-KLH-immunoreactive structures. The current results suggest that protein-bound acrolein is a powerful marker of oxidative damage to protein and support the hypothesis that lipid peroxidation and oxidative damage to protein may play a crucial role in the formation of neurofibrillary tangles and to neuronal death in AD.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-hydroxy-2-nonenal,
http://linkedlifedata.com/resource/pubmed/chemical/Acrolein,
http://linkedlifedata.com/resource/pubmed/chemical/Aldehydes,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
72
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
751-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9930749-Acrolein,
pubmed-meshheading:9930749-Adult,
pubmed-meshheading:9930749-Aged,
pubmed-meshheading:9930749-Aldehydes,
pubmed-meshheading:9930749-Alzheimer Disease,
pubmed-meshheading:9930749-Antibody Specificity,
pubmed-meshheading:9930749-Biological Markers,
pubmed-meshheading:9930749-Cysteine Proteinase Inhibitors,
pubmed-meshheading:9930749-Cytoskeleton,
pubmed-meshheading:9930749-Female,
pubmed-meshheading:9930749-Humans,
pubmed-meshheading:9930749-Lipid Peroxidation,
pubmed-meshheading:9930749-Male,
pubmed-meshheading:9930749-Middle Aged,
pubmed-meshheading:9930749-Nerve Degeneration,
pubmed-meshheading:9930749-Neurofibrillary Tangles,
pubmed-meshheading:9930749-Neurons,
pubmed-meshheading:9930749-Oxidative Stress
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Protein-bound acrolein: a novel marker of oxidative stress in Alzheimer's disease.
|
| pubmed:affiliation |
Department of Neurology and Neuroscience, Cornell University Medical College at Burke Medical Research Institute, White Plains, New York 10605, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|