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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-2-25
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pubmed:abstractText |
To define the early biological effect of in vivo introduction of the PDGF-B gene on the healing of ligaments, a HVJ-liposome suspension containing platelet-derived growth factor (PDGF)-B cDNA was injected directly into the injured patellar ligament of 14-week-old male Wistar rats. Rats were killed at 1, 4 and 8 weeks for the morphological analysis of angiogenesis by laminin immunohistochemistry and of collagen deposition by Masson's Trichrome staining and collagen I immunohistochemistry. PDGF-B gene transfer caused the enhanced expression of PDGF in healing ligament up to 4 weeks after transfection, leading to an initial promotion of angiogenesis and subsequent enhanced collagen deposition in the wound. Enhanced and accelerated matrix synthesis in the PDGF-B gene introduced healing ligament suggests that this gene transfer technique may be a potentially useful tool for improving soft tissue repair.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0969-7128
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1165-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9930316-Animals,
pubmed-meshheading:9930316-Collagen,
pubmed-meshheading:9930316-Gene Expression,
pubmed-meshheading:9930316-Gene Therapy,
pubmed-meshheading:9930316-Gene Transfer Techniques,
pubmed-meshheading:9930316-Genetic Vectors,
pubmed-meshheading:9930316-Immunohistochemistry,
pubmed-meshheading:9930316-Laminin,
pubmed-meshheading:9930316-Liposomes,
pubmed-meshheading:9930316-Male,
pubmed-meshheading:9930316-Neovascularization, Physiologic,
pubmed-meshheading:9930316-Patellar Ligament,
pubmed-meshheading:9930316-Platelet-Derived Growth Factor,
pubmed-meshheading:9930316-Proto-Oncogene Proteins,
pubmed-meshheading:9930316-Proto-Oncogene Proteins c-sis,
pubmed-meshheading:9930316-Rats,
pubmed-meshheading:9930316-Rats, Wistar,
pubmed-meshheading:9930316-Respirovirus,
pubmed-meshheading:9930316-Wound Healing
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pubmed:year |
1998
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pubmed:articleTitle |
Early biological effect of in vivo gene transfer of platelet-derived growth factor (PDGF)-B into healing patellar ligament.
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pubmed:affiliation |
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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