Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-9
pubmed:abstractText
Depth-dependent fluorescence quenching in membranes is playing an increasingly important role in the determination of the low resolution structure of membrane proteins. This paper presents a graphical way of visualizing membrane quenching caused by lipid-attached bromines or spin labels with the help of a depth-dependent fluorescence quenching profile. Two methods are presently available to extract information on membrane penetration from quenching: the parallax method (PM; ) and distribution analysis (DA; A. S. Biophys. J. 64:290a (Abstr.); A. S. Methods Enzymol. 278:462-473). Analysis of various experimental and simulated data by these two methods is presented. The effects of uncertainty in the local concentration of quenching lipids (due to protein shielding or nonideality in lipid mixing), the existence of multiple conformations of membrane-bound protein, incomplete binding, and uncertainty in the fluorescence in nonquenching lipid are described. Regardless of the analytical form of the quenching profile (Gaussian function for DA or truncated parabola for PM), it has three primary characteristics: position on the depth scale, area, and width. The most important result, not surprisingly, is that one needs three fitting parameters to describe the quenching. This will keep the measures of the quenching profile independent of each other resulting in the reduction of systematic errors in depth determination. This can be achieved by using either DA or a suggested modification of the PM that introduces a third parameter related to quenching efficiency. Because DA utilizes a smooth fitting function, it offers an advantage for the analysis of deeply penetrating probes, where the effects of transleaflet quenching should be considered.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-1378757, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-1510921, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-1554721, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-1606155, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-1731926, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-1992480, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-2036418, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-2069956, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-2139793, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-2161684, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-2271606, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-3026475, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-3030403, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-3593689, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-3700412, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-3790588, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-6854644, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-7492549, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-7547874, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-7612625, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-7669893, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-7819486, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-7849052, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8136371, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8334124, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8399232, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8527665, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8592783, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8780526, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8842228, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-8867339, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-9048567, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-9154930, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929496-9170327
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
946-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Analysis of protein and peptide penetration into membranes by depth-dependent fluorescence quenching: theoretical considerations.
pubmed:affiliation
Department of Physiology and Biophysics, University of California-Irvine, Irvine, California 92697-4560 USA. ladokhin@uci.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.