| pubmed-article:9929476 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0599844 | lld:lifeskim |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0242849 | lld:lifeskim |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0449445 | lld:lifeskim |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0750540 | lld:lifeskim |
| pubmed-article:9929476 | lifeskim:mentions | umls-concept:C0205485 | lld:lifeskim |
| pubmed-article:9929476 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9929476 | pubmed:dateCreated | 1999-3-9 | lld:pubmed |
| pubmed-article:9929476 | pubmed:abstractText | Atomic force microscopy is one of the few techniques that allow analysis of biological recognition processes at the single-molecule level. A major limitation of this approach is the nonspecific interaction between the force sensor and substrate. We have modeled the nonspecific interaction by looking at the interaction potential between a conical Si3N4 tip with a spherical end face and a mica surface in solution, using DLVO (Derjaguin, Landau, Verwey, Overbeek) theory and numerical calculations. Insertion of the tip-sample potential in a simulation of an approach-retract cycle of the cantilever gives the well-known force-distance curve. Simulating a force-distance curve at low salt concentration predicts a discrete hopping of the tip, caused by thermal fluctuations. This hopping behavior was observed experimentally and gave rise to a novel approach to making measurements in adhesion mode that essentially works in the repulsive regime. The distance between tip and sample will still be small enough to allow spacer-involved specific interactions, and the percentage of nonspecific interactions of the bare tip with the mica is minimized. We have validated this physical model by imaging intercellular adhesion molecule 1 (ICAM-1) antigen with a tip functionalized with anti-ICAM-1 antibody. The measurement demonstrated that a significant decrease in the number of nonspecific interactions was realized, and the topographical image quality and the specific bonding capability of the tip were not affected. | lld:pubmed |
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| pubmed-article:9929476 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9929476 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9929476 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9929476 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9929476 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9929476 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9929476 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9929476 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9929476 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:9929476 | pubmed:author | pubmed-author:De GroothB... | lld:pubmed |
| pubmed-article:9929476 | pubmed:author | pubmed-author:GreveJJ | lld:pubmed |
| pubmed-article:9929476 | pubmed:author | pubmed-author:KuipersLL | lld:pubmed |
| pubmed-article:9929476 | pubmed:author | pubmed-author:FigdorC GCG | lld:pubmed |
| pubmed-article:9929476 | pubmed:author | pubmed-author:SnelM MMM | lld:pubmed |
| pubmed-article:9929476 | pubmed:author | pubmed-author:WillemsenO... | lld:pubmed |
| pubmed-article:9929476 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9929476 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:9929476 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9929476 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9929476 | pubmed:pagination | 716-24 | lld:pubmed |
| pubmed-article:9929476 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:meshHeading | pubmed-meshheading:9929476-... | lld:pubmed |
| pubmed-article:9929476 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:9929476 | pubmed:articleTitle | A physical approach to reduce nonspecific adhesion in molecular recognition atomic force microscopy. | lld:pubmed |
| pubmed-article:9929476 | pubmed:affiliation | Department of Applied Physics, Applied Optics Group, University of Twente, Enschede, The Netherlands. | lld:pubmed |
| pubmed-article:9929476 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9929476 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9929476 | lld:pubmed |
