Linked Life Data

9929476

Source:http://linkedlifedata.com/resource/pubmed/id/9929476

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-9
pubmed:abstractText
Atomic force microscopy is one of the few techniques that allow analysis of biological recognition processes at the single-molecule level. A major limitation of this approach is the nonspecific interaction between the force sensor and substrate. We have modeled the nonspecific interaction by looking at the interaction potential between a conical Si3N4 tip with a spherical end face and a mica surface in solution, using DLVO (Derjaguin, Landau, Verwey, Overbeek) theory and numerical calculations. Insertion of the tip-sample potential in a simulation of an approach-retract cycle of the cantilever gives the well-known force-distance curve. Simulating a force-distance curve at low salt concentration predicts a discrete hopping of the tip, caused by thermal fluctuations. This hopping behavior was observed experimentally and gave rise to a novel approach to making measurements in adhesion mode that essentially works in the repulsive regime. The distance between tip and sample will still be small enough to allow spacer-involved specific interactions, and the percentage of nonspecific interactions of the bare tip with the mica is minimized. We have validated this physical model by imaging intercellular adhesion molecule 1 (ICAM-1) antigen with a tip functionalized with anti-ICAM-1 antibody. The measurement demonstrated that a significant decrease in the number of nonspecific interactions was realized, and the topographical image quality and the specific bonding capability of the tip were not affected.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-19431803, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-19431815, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-7632794, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-7819507, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-8075349, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-8153628, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-8622961, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-9172770, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-9200694, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-9284330, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-9635738, http://linkedlifedata.com/resource/pubmed/commentcorrection/9929476-9788917
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
716-24
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
A physical approach to reduce nonspecific adhesion in molecular recognition atomic force microscopy.
pubmed:affiliation
Department of Applied Physics, Applied Optics Group, University of Twente, Enschede, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't