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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1999-2-16
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pubmed:abstractText |
Pituitary adenylate cyclase-activating polypeptides (PACAP-27 and -38) are neuropeptides of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family. PACAP receptors are expressed in different brain regions including the cerebellum. We used primary culture of rat cerebellar granule neurons to study the effect of PACAP-38 on apoptosis induced by potassium deprivation. We demonstrated that serum and potassium withdrawal induces a mixture of apoptosis and necrosis rather than apoptosis only. We showed that PACAP-38 increased survival of cerebellar neurons in a dose-dependent manner by specifically decreasing the extent of apoptosis estimated by DNA fragmentation. PACAP-38 induced activation of the extracellular signal-regulated kinase (ERK)-type of MAP kinase through a cAMP-dependent pathway. PD98059, an inhibitor of MEK (MAP kinase kinase), completely abolished the anti-apoptotic effect of PACAP-38, suggesting that MAP kinase pathway activation is necessary for PACAP-38 effect.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adcyap1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Hormone
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0077-8923
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
865
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-10
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9928002-Animals,
pubmed-meshheading:9928002-Apoptosis,
pubmed-meshheading:9928002-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9928002-Cells, Cultured,
pubmed-meshheading:9928002-Cerebellum,
pubmed-meshheading:9928002-DNA Fragmentation,
pubmed-meshheading:9928002-Enzyme Inhibitors,
pubmed-meshheading:9928002-Flavonoids,
pubmed-meshheading:9928002-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:9928002-Necrosis,
pubmed-meshheading:9928002-Neurons,
pubmed-meshheading:9928002-Neuropeptides,
pubmed-meshheading:9928002-Neuroprotective Agents,
pubmed-meshheading:9928002-Pituitary Adenylate Cyclase-Activating Polypeptide,
pubmed-meshheading:9928002-Potassium,
pubmed-meshheading:9928002-Protein Kinases,
pubmed-meshheading:9928002-Rats,
pubmed-meshheading:9928002-Receptors, Pituitary Adenylate Cyclase-Activating...,
pubmed-meshheading:9928002-Receptors, Pituitary Hormone
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pubmed:year |
1998
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pubmed:articleTitle |
PACAP-38 protects cerebellar granule cells from apoptosis.
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pubmed:affiliation |
Centre National de la Recherche Scientifique, Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Montpellier, France. journot@ccipe.montp.inserm.fr
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pubmed:publicationType |
Journal Article,
Review
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