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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1999-2-16
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pubmed:abstractText |
The 5'-flanking region (1.5 kb) of the gene coding for the human VIP1/PACAP receptor was isolated, sequenced, and characterized. Transient expression of constructs containing sequentially deleted 5'-flanking sequences of the VIP1/PACAP receptor fused to a luciferase reporter gene showed that this sequence was active as a promoter in the intestinal cancer cell line, HT-29, expressing endogenous VIP1/PACAP receptor. The shortest DNA fragment with significant promoter activity encompassed the region from -205 to +76 bp. Deletion of a CCAAT-box sequence in the construction corresponding to -173 to +76 bp dramatically reduced the promoter activity. The promoter -205 to +76 bp has a housekeeping gene structure without TATA-box. It contains GC-rich regions characterized by potential Sp1 and AP2 sites and some potential regulatory elements, such as CRE and ATF, and a CCAAT-box sequence (-182 to -178) crucial for gene transcription.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0077-8923
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
865
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-63
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9927997-Cloning, Molecular,
pubmed-meshheading:9927997-Female,
pubmed-meshheading:9927997-Genomic Library,
pubmed-meshheading:9927997-Humans,
pubmed-meshheading:9927997-Luciferases,
pubmed-meshheading:9927997-Placenta,
pubmed-meshheading:9927997-Pregnancy,
pubmed-meshheading:9927997-Promoter Regions, Genetic,
pubmed-meshheading:9927997-Receptors, Pituitary Adenylate Cyclase-Activating...,
pubmed-meshheading:9927997-Receptors, Pituitary Hormone,
pubmed-meshheading:9927997-Receptors, Vasoactive Intestinal Peptide,
pubmed-meshheading:9927997-Receptors, Vasoactive Intestinal Polypeptide, Type I,
pubmed-meshheading:9927997-Recombinant Fusion Proteins,
pubmed-meshheading:9927997-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9927997-Sequence Deletion,
pubmed-meshheading:9927997-TATA Box,
pubmed-meshheading:9927997-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Cloning and functional characterization of the human VIP1/PACAP receptor promoter.
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pubmed:affiliation |
Laboratoire de Neuroendocrinologie et Biologie Cellulaire Digestives, Institut National de la Santé et de la Recherche Médicale, INSERM U-410, Faculté de Médecine Xavier Bichat, Paris, France. coucou@bichat.inserm.fr
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pubmed:publicationType |
Journal Article
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