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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-3-5
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pubmed:abstractText |
At the synapse, presynaptic membranes specialized for vesicular traffic are linked to postsynaptic membranes specialized for signal transduction. The mechanisms that connect pre- and postsynaptic membranes into synaptic junctions are unknown. Neuroligins and beta-neurexins are neuronal cell-surface proteins that bind to each other and form asymmetric intercellular junctions. To test whether the neuroligin/beta-neurexin junction is related to synapses, we generated and characterized monoclonal antibodies to neuroligin 1. With these antibodies, we show that neuroligin 1 is synaptic. The neuronal localization, subcellular distribution, and developmental expression of neuroligin 1 are similar to those of the postsynaptic marker proteins PSD-95 and NMDA-R1 receptor. Quantitative immunogold electron microscopy demonstrated that neuroligin 1 is clustered in synaptic clefts and postsynaptic densities. Double immunofluorescence labeling revealed that neuroligin 1 colocalizes with glutamatergic but not gamma-aminobutyric acid (GABA)ergic synapses. Thus neuroligin 1 is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. In addition, the neuroligin/beta-neurexin junction may be involved in the specification of excitatory synapses.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-1621094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-3097029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-7675218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-7695896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-7736595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8207001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8290563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8576240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8778289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8833447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8909549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8929566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8954715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-8985745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9065497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9162013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9278515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9325340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9331349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9352219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9354326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9390515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9448462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9454832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9581761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9620694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9655502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9655504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9927700-9753324
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, Neuronal,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptophysin,
http://linkedlifedata.com/resource/pubmed/chemical/neuroligin 1,
http://linkedlifedata.com/resource/pubmed/chemical/postsynaptic density proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1100-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9927700-Aging,
pubmed-meshheading:9927700-Animals,
pubmed-meshheading:9927700-Brain,
pubmed-meshheading:9927700-COS Cells,
pubmed-meshheading:9927700-Cell Adhesion Molecules,
pubmed-meshheading:9927700-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:9927700-Cerebellum,
pubmed-meshheading:9927700-Embryonic and Fetal Development,
pubmed-meshheading:9927700-Gene Expression Regulation, Developmental,
pubmed-meshheading:9927700-Guanylate Kinase,
pubmed-meshheading:9927700-Hippocampus,
pubmed-meshheading:9927700-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:9927700-Membrane Proteins,
pubmed-meshheading:9927700-Mice,
pubmed-meshheading:9927700-Mice, Inbred C57BL,
pubmed-meshheading:9927700-Microscopy, Immunoelectron,
pubmed-meshheading:9927700-Neocortex,
pubmed-meshheading:9927700-Nerve Tissue Proteins,
pubmed-meshheading:9927700-Pyramidal Cells,
pubmed-meshheading:9927700-Rats,
pubmed-meshheading:9927700-Rats, Wistar,
pubmed-meshheading:9927700-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:9927700-Synapses,
pubmed-meshheading:9927700-Synaptophysin,
pubmed-meshheading:9927700-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses.
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pubmed:affiliation |
Max-Planck-Institut für Experimentelle Medizin, Abteilung Molekulare Neurobiologie, Hermann-Rein-Strasse 3, D-37075 Göttingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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