Linked Life Data

9927338

Source:http://linkedlifedata.com/resource/pubmed/id/9927338

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-2-23
pubmed:abstractText
There is considerable evidence that luteolysis in the cow and other species involves components of the immune system. In this study, we examined the expression of the mRNAs for TNF-alpha, IFN-gamma, IL-1beta, IL-2, and IL-2 receptor (IL-2R) by reverse transcription-polymerase chain reaction (RT-PCR) using bovine-specific primers. Expression was examined in corpora lutea (CL) of the early (day 5), mid (days 11-12), and late (day 18) luteal phase, and at 1, 4, and 24 hours following a luteolytic dose of prostaglandin (PG) F2alpha. Tumor necrosis factor-alpha, IFN-gamma, and IL-1beta mRNAs were detectable by RT-PCR at all stages of the cycle examined. Densitometric intensities of the electrophoresed IFN-gamma PCR products revealed a drop in RNA expression during late diestrus and at one hour of prostaglandin-induced luteolysis (P < 0.05). The mRNA for TNF-alpha seemed to remain constant during the cycle, and rose slightly during luteolysis. Interleukin-1beta mRNA also did not vary during the cycle or during luteolysis. Finally, expression of mRNAs for IL-2 and IL-2 receptor was not evident in CL by the methods employed in this study. These results are the first to describe mRNA expression for TNF-alpha, IFN-gamma, and IL-1beta in the bovine corpus luteum, and support a role for these cytokines in luteal function and regression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1018-21
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Expression of cytokine messenger ribonucleic acids in the bovine corpus luteum.
pubmed:affiliation
Department of Animal Sciences, Ohio State University, Wooster, USA.
pubmed:publicationType
Journal Article, In Vitro