Linked Life Data

9927192

Source:http://linkedlifedata.com/resource/pubmed/id/9927192

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-2-16
pubmed:abstractText
beta-spectrins are crucial for the maintenance of cell shape, the establishment of cell polarity, and the formation of distinct membrane domains. Our strategy for identifying genes important for hepatocyte polarity has been to utilize subtractive hybridization of early embryonic mouse cDNA liver libraries. As a result, we have cloned three isoforms of a novel beta-spectrin elf (embryonic liver beta-fodrin), and here we report the analysis of elf3, the longest isoform (8172 nt). ELF3 comprises 2154 residues with an overall similarity of 89.0% and 95.3% to mouse beta-spectrin (betaSpIIsigma1) at the nucleotide and amino acid level, respectively. ELF3 is characterized by an actin-binding domain, a long repeat domain, and a short regulatory domain remarkable for the absence of a PH domain. Linkage analysis reveals that elf3 maps to mouse chromosome 11 between D11Bir6 and D11Xrf477, a different chromosomal locus from that of the other four spectrin genes. Northern blot analysis utilizing an elf3 3'-UTR probe demonstrates an abundant 9.0-kb transcript in brain, liver, and heart tissues. Western blot with a polyclonal antibody against ELF identifies a 200 kD protein in mouse liver, brain, kidney, and heart tissues. Immunohistochemical studies demonstrate ELF labeling of the basolateral or sinusoidal membranes surface as well as a granular cytoplasmic pattern in hepatocytes. Antisense studies utilizing cultured liver explants show a vital role of elf3 in hepatocyte differentiation and intrahepatic bile duct formation. The differential expression, tissue localization, and functional studies demonstrate the importance of elf3 in modulating interactions between various components of the cytoskeleton proteins controlling liver and bile duct development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9927192-Amino Acid Sequence, pubmed-meshheading:9927192-Animals, pubmed-meshheading:9927192-Bile Ducts, Intrahepatic, pubmed-meshheading:9927192-Blotting, Western, pubmed-meshheading:9927192-Cell Division, pubmed-meshheading:9927192-Chromosome Mapping, pubmed-meshheading:9927192-Cloning, Molecular, pubmed-meshheading:9927192-DNA, Complementary, pubmed-meshheading:9927192-DNA-Binding Proteins, pubmed-meshheading:9927192-Immunohistochemistry, pubmed-meshheading:9927192-Liver, pubmed-meshheading:9927192-Mice, pubmed-meshheading:9927192-Mice, Inbred ICR, pubmed-meshheading:9927192-Molecular Sequence Data, pubmed-meshheading:9927192-Oligonucleotides, Antisense, pubmed-meshheading:9927192-Proto-Oncogene Proteins, pubmed-meshheading:9927192-RNA, Messenger, pubmed-meshheading:9927192-Sequence Homology, Amino Acid, pubmed-meshheading:9927192-Spectrin, pubmed-meshheading:9927192-Transcription Factors
pubmed:year
1999
pubmed:articleTitle
Elf3 encodes a novel 200-kD beta-spectrin: role in liver development.
pubmed:affiliation
Laboratory of Developmental Molecular Biology, Department of Veterans Affairs, Washington, DC 20422, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.