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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-2-26
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pubmed:abstractText |
We have previously shown that Sp1-mediated transcription is stimulated by Rb and repressed by cyclin D1. The stimulation of Sp1 transcriptional activity by Rb is conferred, in part, through a direct interaction with the TBP-associated factor TAF(II)250. Here we investigated the mechanism(s) through which cyclin D1 represses Sp1. We examined the ability of cyclin D1 to regulate transcription mediated by Gal4-Sp1 fusion proteins, which contain the Gal4 DNA-binding domain and Sp1 trans-activation domain(s). The domain of Sp1 sufficient to confer repression by cyclin D1 was mapped to a region important for interaction with TAF(II)110. We further demonstrate that TAF(II)250-cyclin D1 complexes can be immunoprecipitated from mammalian and baculovirus-infected insect cells and that recombinant GST-TAF(II)250 (amino acids 1-434) associates with cyclin D1 in vitro. Moreover, the overexpression of Rb or CDK4 reduced the level of TAF(II)250-cyclin D1 complex. The amino terminus of cyclin D1 (amino acids 1-100) was sufficient for association with TAF(II)250 and for repressing Sp1-mediated transcription. Taken together, the results suggest that cyclin D1 may regulate transcription by interacting directly or indirectly with TAF(II)250.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDK4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Binding Protein Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-Box Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/TATA-binding protein associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIID,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-47
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9926939-3T3 Cells,
pubmed-meshheading:9926939-Animals,
pubmed-meshheading:9926939-Cell Line,
pubmed-meshheading:9926939-Cyclin D1,
pubmed-meshheading:9926939-Cyclin-Dependent Kinase 4,
pubmed-meshheading:9926939-Cyclin-Dependent Kinases,
pubmed-meshheading:9926939-DNA-Binding Proteins,
pubmed-meshheading:9926939-Gene Expression Regulation,
pubmed-meshheading:9926939-Humans,
pubmed-meshheading:9926939-Mice,
pubmed-meshheading:9926939-Mutagenesis,
pubmed-meshheading:9926939-Nuclear Proteins,
pubmed-meshheading:9926939-Proto-Oncogene Proteins,
pubmed-meshheading:9926939-Recombinant Fusion Proteins,
pubmed-meshheading:9926939-Retinoblastoma Protein,
pubmed-meshheading:9926939-Sp1 Transcription Factor,
pubmed-meshheading:9926939-Spodoptera,
pubmed-meshheading:9926939-TATA Box,
pubmed-meshheading:9926939-TATA-Binding Protein Associated Factors,
pubmed-meshheading:9926939-TATA-Box Binding Protein,
pubmed-meshheading:9926939-Transcription, Genetic,
pubmed-meshheading:9926939-Transcription Factor TFIID,
pubmed-meshheading:9926939-Transcription Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Cyclin D1 associates with the TBP-associated factor TAF(II)250 to regulate Sp1-mediated transcription.
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pubmed:affiliation |
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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