Linked Life Data

9926763

Source:http://linkedlifedata.com/resource/pubmed/id/9926763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-4-1
pubmed:abstractText
COMMERCIAL PREPARATIONS OF HUMAN DEMINERALIZED freeze-dried bone allograft (DFDBA) vary in their ability to induce new bone formation. This study tested the hypothesis that inactive DFDBA can be used as an effective carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2). Two batches of active DFDBA were used as controls. Two batches of DFDBA, previously shown to be inactive, were treated with vehicle or with 5 or 20 microg rhBMP-2 and implanted into the calf muscle of male Nu/Nu (nude) mice. Each mouse received one implant in each hind limb, both of which were of the same formulation, resulting in 8 groups of 4 mice per group: active DFDBA batch A, active DFDBA batch B, inactive DFDBA batch A, inactive DFDBA batch B, inactive DFDBA batch A plus 5 microg rhBMP-2, inactive DFDBA batch A plus 20 microg rhBMP-2, inactive DFDBA batch B plus 5 microg rhBMP-2, and inactive DFDBA batch B plus 20 microg rhBMP-2. After 56 days, the implants were removed and histologically examined. A semiquantitative bone induction index was calculated based on the amount of new bone covering each histological section. Histomorphometry was also used to evaluate the area of new bone formed and the area of residual implant material. The results showed that active DFDBA induces new bone formation, whereas inactive DFDBA does not. Addition of rhBMP-2 to inactive DFDBA results in new bone formation with a bone induction index comparable to that of active DFDBA. Histomorphometric analysis, however, revealed that the rhBMP-2-containing implants caused a dose-dependent increase in new bone area that exceeded that induced by active DFDBA. At the highest concentration of rhBMP-2, bone formation was exuberant. rhBMP-2 also caused the resorption of residual implant material to levels comparable to that seen in sites treated with active DFDBA, suggesting that this growth factor may regulate resorptive cells either directly or indirectly. This study shows that addition of rhBMP-2 to inactive DFDBA provides reproducible, consistent bone induction, and suggests that inactive commercial preparations may contain inadequate amounts of BMP to cause bone induction compared to active preparations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3492
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1337-45
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9926763-Animals, pubmed-meshheading:9926763-Bone Marrow, pubmed-meshheading:9926763-Bone Morphogenetic Protein 2, pubmed-meshheading:9926763-Bone Morphogenetic Proteins, pubmed-meshheading:9926763-Bone Resorption, pubmed-meshheading:9926763-Bone Transplantation, pubmed-meshheading:9926763-Cartilage, pubmed-meshheading:9926763-Connective Tissue, pubmed-meshheading:9926763-Dose-Response Relationship, Drug, pubmed-meshheading:9926763-Freeze Drying, pubmed-meshheading:9926763-Humans, pubmed-meshheading:9926763-Male, pubmed-meshheading:9926763-Mice, pubmed-meshheading:9926763-Mice, Nude, pubmed-meshheading:9926763-Muscle, Skeletal, pubmed-meshheading:9926763-Osteogenesis, pubmed-meshheading:9926763-Pharmaceutical Vehicles, pubmed-meshheading:9926763-Recombinant Proteins, pubmed-meshheading:9926763-Transforming Growth Factor beta, pubmed-meshheading:9926763-Transplantation, Homologous
pubmed:year
1998
pubmed:articleTitle
Addition of human recombinant bone morphogenetic protein-2 to inactive commercial human demineralized freeze-dried bone allograft makes an effective composite bone inductive implant material.
pubmed:affiliation
Department of Periodontics, University of Texas Health Science Center, San Antonio 78284-7774, USA. Schwartz@uthsca.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't