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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1999-3-5
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pubmed:databankReference |
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pubmed:abstractText |
Tenascin-R is an extracellular matrix protein expressed exclusively in the central nervous system where it is thought to play a relevant role in regulating neurite outgrowth. We have i) cloned the cDNA of the rat tenascin-R 5' region; ii) defined its genomic organization, obtaining the sequence of two novel untranslated exons; iii) mapped the gene to rat chromosome 13q23 and suggested a previously unreported synteny between rat chromosome 13q23, human chromosome 1q24, and mouse chromosome 4E; and iv) sequenced and characterized the elements responsible for its neural cell-restricted transcription. We found that two discrete regions of the rat gene (the first in the proximal promoter, the second in the first exon) are independently able to activate to a high degree the transcription of a reporter gene in either human or rat neuroblastoma cell lines but not in other cell lines. Based on this observation, we re-evaluated the arrangement of transcriptionally active regions in the human tenascin-R gene we recently cloned and found that the human gene also contains an exon sequence able to initiate and sustain transcription independently of promoter sequences.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0301-0171
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-23
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9925948-5' Untranslated Regions,
pubmed-meshheading:9925948-Animals,
pubmed-meshheading:9925948-Blotting, Northern,
pubmed-meshheading:9925948-Cell Adhesion Molecules,
pubmed-meshheading:9925948-Cerebellum,
pubmed-meshheading:9925948-Chromosome Mapping,
pubmed-meshheading:9925948-Cloning, Molecular,
pubmed-meshheading:9925948-DNA, Complementary,
pubmed-meshheading:9925948-DNA Primers,
pubmed-meshheading:9925948-Exons,
pubmed-meshheading:9925948-Extracellular Matrix Proteins,
pubmed-meshheading:9925948-Gene Expression,
pubmed-meshheading:9925948-Genes, Reporter,
pubmed-meshheading:9925948-Humans,
pubmed-meshheading:9925948-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9925948-Molecular Sequence Data,
pubmed-meshheading:9925948-Neuroblastoma,
pubmed-meshheading:9925948-Promoter Regions, Genetic,
pubmed-meshheading:9925948-RNA, Messenger,
pubmed-meshheading:9925948-Rats,
pubmed-meshheading:9925948-Sequence Homology, Nucleic Acid,
pubmed-meshheading:9925948-Tenascin,
pubmed-meshheading:9925948-Transcriptional Activation,
pubmed-meshheading:9925948-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Rat tenascin-R gene: structure, chromosome location and transcriptional activity of promoter and exon 1.
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pubmed:affiliation |
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro (IST)/Advanced Biotechnology Center (CBA), Genoa (Italy). leprini@ermes.cba.unige.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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