Linked Life Data

9925751

Source:http://linkedlifedata.com/resource/pubmed/id/9925751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-15
pubmed:abstractText
The human A and B subunits of nucleoside diphosphate kinase (NDP kinase), encoded by the nm23-H1 and nm23-H2 genes, respectively, associate as homo- or heterohexamers to be catalytically active for the synthesis of nucleoside triphosphates. Despite 88% identity, they appear to possess specific functions. The nm23-H1 gene is implicated in tumor progression and metastasis, and the nm23-H2 gene product is a transcription factor for c-myc. To determine if these distinct functions reflect different subcellular localizations, the distribution of the A and B NDP kinases was analyzed by immunocytofluorescence microscopy in human breast cancer cell lines (MCF-7 and MDA-MB-231) using highly specific polyclonal and monoclonal antibodies. Interphasic cells exhibited a granular and filamentous cytoplasmic staining, particularly intense around nuclei, with both anti-NDP kinase A and B antibodies. The filamentous component observed with either anti-A or anti-B antibodies was altered in parallel to tubulin labeling with compounds interacting with microtubules, such as taxol and colchicine. Confirming published biochemical data, a partial colocalization with the vimentin network was observed in the MDA-231 cell line. A nuclear and nucleolar localization of NDP kinase B was shown by confocal microscopy which was not observed with the A enzyme. In dividing cells, NDP kinase labeling was punctiform and was not colocalized with the mitotic spindle. In conclusion, the A and B NDP kinases are similarly distributed in cytosol, associated partly to microtubules supporting a role in nucleotide channeling. Only the B enzyme is present in nuclei in accord with its role as a DNA binding protein. Their altered localization in dividing cells suggests colocalization with yet unidentified structures which are not intermediate filament aggregates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-4827
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
246
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-67
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9925751-Animals, pubmed-meshheading:9925751-Antibody Specificity, pubmed-meshheading:9925751-Breast Neoplasms, pubmed-meshheading:9925751-Cell Division, pubmed-meshheading:9925751-Cell Nucleus, pubmed-meshheading:9925751-Cytoskeleton, pubmed-meshheading:9925751-Female, pubmed-meshheading:9925751-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9925751-Humans, pubmed-meshheading:9925751-Interphase, pubmed-meshheading:9925751-Microtubules, pubmed-meshheading:9925751-Mitosis, pubmed-meshheading:9925751-Monomeric GTP-Binding Proteins, pubmed-meshheading:9925751-NM23 Nucleoside Diphosphate Kinases, pubmed-meshheading:9925751-Nucleoside-Diphosphate Kinase, pubmed-meshheading:9925751-Rabbits, pubmed-meshheading:9925751-Transcription Factors, pubmed-meshheading:9925751-Tumor Cells, Cultured, pubmed-meshheading:9925751-Vimentin
pubmed:year
1999
pubmed:articleTitle
Cytoskeletal association of the A and B nucleoside diphosphate kinases of interphasic but not mitotic human carcinoma cell lines: specific nuclear localization of the B subunit.
pubmed:affiliation
INSERM U402, CHU Saint-Antoine, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't