Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-24
pubmed:abstractText
A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extraction of nucleic acid from an aliquot of an overnight culture of feces in EC (modified) broth. The detection limit of the assay for both fecal samples and pure cultures was between 18 and 37 genome equivalents. stx1 and hlyA were the most commonly encountered virulence factors.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-1476425, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-1629337, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-1639518, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-2056066, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-3299029, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-6149247, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-7538509, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-7559976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-7868227, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8005211, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8202028, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8263203, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8406796, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8408571, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8446472, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8636941, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8789031, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8877329, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-8900070, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9041407, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9140657, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9157149, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9172336, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9172396, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9199437, http://linkedlifedata.com/resource/pubmed/commentcorrection/9925634-9466788
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
868-72
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR.
pubmed:affiliation
Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, Camden, New South Wales 2570, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't