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rdf:type |
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lifeskim:mentions |
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0085112,
umls-concept:C0085243,
umls-concept:C0086418,
umls-concept:C0086597,
umls-concept:C0301944,
umls-concept:C0442335,
umls-concept:C0443252,
umls-concept:C0751781,
umls-concept:C0882849,
umls-concept:C1160185
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pubmed:issue |
5402
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pubmed:dateCreated |
1999-2-11
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pubmed:abstractText |
Efficient gene transfer into human hematopoietic stem cells (HSCs) is an important goal in the study of the hematopoietic system as well as for gene therapy of hematopoietic disorders. A lentiviral vector based on the human immunodeficiency virus (HIV) was able to transduce human CD34+ cells capable of stable, long-term reconstitution of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. High-efficiency transduction occurred in the absence of cytokine stimulation and resulted in transgene expression in multiple lineages of human hematopoietic cells for up to 22 weeks after transplantation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0036-8075
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
682-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9924027-Animals,
pubmed-meshheading:9924027-Antigens, CD34,
pubmed-meshheading:9924027-Bone Marrow Cells,
pubmed-meshheading:9924027-Cell Division,
pubmed-meshheading:9924027-Cell Survival,
pubmed-meshheading:9924027-Colony-Forming Units Assay,
pubmed-meshheading:9924027-Gene Expression,
pubmed-meshheading:9924027-Gene Transfer Techniques,
pubmed-meshheading:9924027-Genetic Vectors,
pubmed-meshheading:9924027-Green Fluorescent Proteins,
pubmed-meshheading:9924027-HIV,
pubmed-meshheading:9924027-Hematopoiesis,
pubmed-meshheading:9924027-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:9924027-Hematopoietic Stem Cells,
pubmed-meshheading:9924027-Humans,
pubmed-meshheading:9924027-Leukemia Virus, Murine,
pubmed-meshheading:9924027-Luminescent Proteins,
pubmed-meshheading:9924027-Mice,
pubmed-meshheading:9924027-Mice, Inbred NOD,
pubmed-meshheading:9924027-Mice, SCID,
pubmed-meshheading:9924027-Promoter Regions, Genetic,
pubmed-meshheading:9924027-Transfection,
pubmed-meshheading:9924027-Transgenes
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pubmed:year |
1999
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pubmed:articleTitle |
Transduction of human CD34+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors.
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pubmed:affiliation |
Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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