Linked Life Data

9923724

Source:http://linkedlifedata.com/resource/pubmed/id/9923724

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1999-4-22
pubmed:abstractText
We used dynamic in vivo 31P magnetic resonance spectroscopy to noninvasively study the metabolism of glycerol by the liver in living rats, as a means of detecting subtle metabolic changes induced by chronic ethanol consumption. Rats subjected to chronic ethanol consumption and their pair-fed controls were given a metabolic load of glycerol (0.75 or 1.3 mL glycerol x kg body mass(-1), i.p. or i.v) under normoxic or hyperoxic (98% O2) conditions. Changes in the level of glycerol 3-phosphate were followed in situ by monitoring the hepatic 31P phosphomonoester resonance every 7 or 13 min for up to 330 min. When challenged with a large dose of glycerol, chronic ethanol-treated rats exhibited less accumulation of glycerol 3-phosphate than controls, independent of the route of administration of the glycerol or whether the two groups were fasted or fed. For example, 1.3 mL glycerol x kg(-1) i.v. under normoxic conditions resulted in a two-fold increase in phosphomonoester in ethanol-treated rats compared with a five-fold increase in controls. The ethanol-treated rats also showed a slower rate of phosphorylation of glycerol and slower oxidation of glycerol 3-phosphate than controls, indicating decreased activities of the glycerol kinase and glycerol 3-phosphate dehydrogenase steps, and hence slower glycerol utilization. The rate of glycerol utilization was dose and oxygen concentration dependent. Kinetic analysis indicated that the chronic ethanol-induced decrease in the glycerol 3-phosphate dehydrogenase reaction was due to a decreased rate of NADH reoxidation in the liver, likely owing to a decrease in oxygen supply or utilization in the ethanol-treated rats. These observations support the hypothesis of pre-existing hypoxia in rat liver after chronic ethanol administration. This study demonstrates the utility of dynamic in vivo 31P magnetic resonance spectroscopy in following the metabolism of a glycerol load as a sensitive, nonperturbing, and potentially clinically applicable test of liver function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0829-8211
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
542-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9923724-Adenosine Triphosphate, pubmed-meshheading:9923724-Alcoholism, pubmed-meshheading:9923724-Animals, pubmed-meshheading:9923724-Diet, pubmed-meshheading:9923724-Eating, pubmed-meshheading:9923724-Energy Metabolism, pubmed-meshheading:9923724-Ethanol, pubmed-meshheading:9923724-Fasting, pubmed-meshheading:9923724-Glycerol, pubmed-meshheading:9923724-Glycerol Kinase, pubmed-meshheading:9923724-Glycerolphosphate Dehydrogenase, pubmed-meshheading:9923724-Glycerophosphates, pubmed-meshheading:9923724-Liver, pubmed-meshheading:9923724-Liver Function Tests, pubmed-meshheading:9923724-Magnetic Resonance Spectroscopy, pubmed-meshheading:9923724-Male, pubmed-meshheading:9923724-NAD, pubmed-meshheading:9923724-Oxidation-Reduction, pubmed-meshheading:9923724-Oxygen, pubmed-meshheading:9923724-Phosphorus Isotopes, pubmed-meshheading:9923724-Phosphorylation, pubmed-meshheading:9923724-Rats, pubmed-meshheading:9923724-Rats, Wistar
pubmed:year
1998
pubmed:articleTitle
Chronic ethanol administration alters hepatic rates of glycerol phosphorylation and glycerol 3-phosphate oxidation: a dynamic in vivo 31P magnetic resonance spectroscopy study.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of Guelph, ON, Canada. brauer@chembio.uoguelph.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't