Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-2-11
pubmed:databankReference
pubmed:abstractText
The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular protein Naf1 (Nef-associated factor 1), which associated with Nef in the yeast two-hybrid system and pull-down assay. The Naf1 gene generates two isoforms (Naf1alpha and beta) containing four coiled-coil structures. The Naf1 mRNA is ubiquitously expressed in human tissues with strong expression in peripheral blood lymphocytes and spleen. Naf1 overexpression increased cell surface CD4 expression. Nef suppressed this Naf1-induced augmentation of CD4 expression, providing a novel mode of Nef action in CD4 down-regulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
442
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-8
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed-meshheading:9923610-Amino Acid Sequence, pubmed-meshheading:9923610-Antigens, CD4, pubmed-meshheading:9923610-Base Sequence, pubmed-meshheading:9923610-Cell Line, pubmed-meshheading:9923610-Cell Membrane, pubmed-meshheading:9923610-Cloning, Molecular, pubmed-meshheading:9923610-DNA, Complementary, pubmed-meshheading:9923610-DNA-Binding Proteins, pubmed-meshheading:9923610-Down-Regulation, pubmed-meshheading:9923610-Female, pubmed-meshheading:9923610-Gene Expression, pubmed-meshheading:9923610-Gene Products, nef, pubmed-meshheading:9923610-Genes, nef, pubmed-meshheading:9923610-HIV-1, pubmed-meshheading:9923610-Humans, pubmed-meshheading:9923610-Male, pubmed-meshheading:9923610-Molecular Sequence Data, pubmed-meshheading:9923610-Pregnancy, pubmed-meshheading:9923610-RNA, Messenger, pubmed-meshheading:9923610-Saccharomyces cerevisiae, pubmed-meshheading:9923610-Tissue Distribution, pubmed-meshheading:9923610-nef Gene Products, Human Immunodeficiency Virus
pubmed:year
1999
pubmed:articleTitle
Identification and cloning of a novel cellular protein Naf1, Nef-associated factor 1, that increases cell surface CD4 expression.
pubmed:affiliation
Department of Microbiology, School of Medicine, Tokyo Medical and Dental University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't